Effects of the Distance from the Sample to Cell Layer in Three-dimensional Tissue Model

To collect basic data for determining cytotoxicity with a three-dimensional tissue model that simulates the living body, direct contact between the sample and cell layer through collagen was compared with collagen insertion between the sample and cell layer using six dental monomers (Bis-GMA, UDMA, Bis-MPEPP, D-2.6E, TEGDMA, and HEMA). Cytotoxicity levels were different for some monomers in the two tissue models, suggesting that the tissue model design influences cytotoxicity. Tissue models for preclinical studies on dental biomaterials will become more strongly desired. Thus, such models should be improved and established for the biological evaluation of dental biomaterials.