Purification and partial characterisation of phenoloxidase from the colonial ascidian Botryllus schlosseri

Purification and partial characterisation of phenoloxidase from the colonial ascidian Botryllus schlosseri

Phenoloxidase (PO) from the colonial ascidian Botryllus schlosseri was purified using two different chromatographic strategies. A three-step purification was developed in order to maintain enzyme activity, whereas an easier purification procedure was adopted to obtain enough PO for the production of...

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Veröffentlicht in:Marine biology 1999-12, Vol.135 (3), p.483-488
Hauptverfasser: FRIZZO, A, GUIDOLIN, L, BALLARIN, L, SABBADIN, A
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Botryllus schlosseri
Cnidaria. Ctenaria
Enzymatic activity
Enzymes
Fundamental and applied biological sciences. Psychology
Invertebrates
Marine
Marine biology
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Zusammenfassung:Phenoloxidase (PO) from the colonial ascidian Botryllus schlosseri was purified using two different chromatographic strategies. A three-step purification was developed in order to maintain enzyme activity, whereas an easier purification procedure was adopted to obtain enough PO for the production of specific polyclonal antibodies.
ISSN:0025-3162
1432-1793
DOI:10.1007/s002270050648