Genetic profile of Mycobacterium tuberculosis and treatment outcomes in human pulmonary tuberculosis in Tanzania
Information on the different spoligotype families of Mycobacterium tuberculosis in Tanzania is limited, and where available, restricted to small geographical areas. This article describes the genetic profile of M. tuberculosis across Tanzania and suggests how spoligotype families might affect drug r...
Gespeichert in:
Veröffentlicht in: | Tanzania journal of health research 2014-04, Vol.16 (2), p.58-69 |
---|---|
Hauptverfasser: | , , , , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Information on the different spoligotype families of Mycobacterium
tuberculosis in Tanzania is limited, and where available, restricted
to small geographical areas. This article describes the genetic profile
of M. tuberculosis across Tanzania and suggests how spoligotype
families might affect drug resistance and treatment outcomes for smear
positive pulmonary tuberculosis patients in Tanzania. We conducted the
study from 2006 to 2008, and the isolates were obtained from samples
collected under the routine drug resistance surveillance system. The
isolates were from specimens collected from 2001 to 2007, and stored at
the Central and Reference Tuberculosis Laboratory. A total of 487
isolates from 23 regions in the country were spoligotyped. We were able
to retrieve clinical information for 446 isolates only. Out of the 487
isolates spoligotyped, 195(40.0%) belonged to the Central Asian (CAS)
family, 84 (17.5%) to the Latin American Mediterranean (LAM) family, 49
(10.1%) to the East-African Indian (EAI) family, and 33 (6.8%) to the
Beijing family. Other isolates included 1 (0.2%) for H37Rv, 10 (2.1%)
for Haarlem, 4 (0.8%) for S family, 58 (11.9%) for T family and 52
(10.7%) for unclassified. No spoligotype patterns were consistent with
M. bovis. Regarding treatment outcomes, the cure rate was 80% with no
significant variation among the spoligotype families. The overall level
of MDR TB was 2.5% (3/121), with no significant difference among the
spoligotype families. All Beijing strains (11.8%, 30/254) originated
from the Eastern and Southern zones of the country, of which 80% were
from Dar es Salaam. Isolates from the CAS and T families were reported
disproportionately from the Eastern-Southern zone, and EAI and LAM
families from the Northern-Lake zones but the difference was not
statistically significant. Five isolates were identified as
non-tuberculous Mycobacteria. In conclusion, M. tuberculosis isolates
from pulmonary tuberculosis cases in Tanzania were classified mostly
within the CAS, LAM, and EAI and T families, while the Beijing family
comprised about 7% isolates only. Consistently good treatment outcomes
were recorded across these spoligotype families. The proportion of drug
resistance strains was low. The findings also suggest variation of
spoligotype families with varying geographical localities within the
country, and identify this area for further research to confirm this
finding. |
---|---|
ISSN: | 1821-6404 1821-9241 |
DOI: | 10.4314/thrb.v16i2.1 |