Biosynthesis and secretion of recombinant human growth hormone in Pichia pastoris

Mature human growth hormone (hGH) cDNA was cloned by homologous recombination into the yeast Pichia pastoris genome. The hGH gene expression was placed under the control of the methanol-inducible alcohol oxidase 1 (AOX1) gene promoter and the Saccharomyces cerevisiae alpha -factor signal sequence to...

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Veröffentlicht in:	Biotechnology letters 2000-01, Vol.22 (2), p.109-114
Hauptverfasser:	ECAMILLA-TREVINO, L. L, VIADER-SALVADO, J. M, BARRERA-SALDANA, H. A, GUERRERO-OLAZARAN, M
Format:	Artikel
Sprache:	eng
Schlagworte:	AOX1 gene Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics growth hormone Health. Pharmaceutical industry Hormones. Growth factors Industrial applications and implications. Economical aspects Methods. Procedures. Technologies Modification of gene expression level Pichia pastoris Production of active biomolecules Saccharomyces cerevisiae
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Zusammenfassung:	Mature human growth hormone (hGH) cDNA was cloned by homologous recombination into the yeast Pichia pastoris genome. The hGH gene expression was placed under the control of the methanol-inducible alcohol oxidase 1 (AOX1) gene promoter and the Saccharomyces cerevisiae alpha -factor signal sequence to direct the secretion of recombinant human growth hormone (rhGH) into the growth medium. O sub(2)-limited induction of recombinant yeast strains in shake tubes with 3 ml of culture medium produced up to 11 mg rhGH 1 super(-1), while high cell density cultures using a 2-1 bioreactor produced about 49 mg rhGH 1 super(-1) achieving 40% of total protein of the culture medium supernatant.
ISSN:	0141-5492 1573-6776
DOI:	10.1023/A:1005675920451