Solid-State NMR of PEGylated Proteins

PEGylated proteins are widely used in biomedicine but, in spite of their importance, no atomic‐level information is available since they are generally resistant to structural characterization approaches. PEGylated proteins are shown here to yield highly resolved solid‐state NMR spectra, which allows assessment of the structural integrity of proteins when PEGylated for therapeutic or diagnostic use. PEGging down proteins: PEGylated proteins are an elusive target for structure elucidation since they are usually too large for solution NMR and tend not to crystallize. Pelleted PEGylated proteins were demonstrated to yield high‐resolution spectra that are suitable for structural characterization and allow the tracking of minor structural changes as a result of PEGylation.