Comprehensive analysis of classical and newly described staphylococcal superantigenic toxin genes in Staphylococcus aureus isolates

We describe a comprehensive detection system for 18 kinds of classical and newly described staphylococcal superantigenic toxin genes using four sets of multiplex PCR. Superantigenic toxin genotyping of Staphylococcus aureus for 69 food poisoning isolates and 97 healthy human nasal swab isolates reve...

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Veröffentlicht in:	FEMS microbiology letters 2005-05, Vol.246 (2), p.191-198
Hauptverfasser:	Omoe, Katsuhiko, Hu, Dong-Liang, Takahashi-Omoe, Hiromi, Nakane, Akio, Shinagawa, Kunihiro
Format:	Artikel
Sprache:	eng
Schlagworte:	bacterial toxins Bacterial Typing Techniques Bacteriology Biological and medical sciences DNA Transposable Elements - genetics Enterotoxin Enterotoxins - classification Enterotoxins - genetics Food contamination food pathogens Food poisoning Fundamental and applied biological sciences. Psychology Genes Genotype Genotypes Genotyping Humans Microbiology Mobile genetic elements Multiplex PCR Nose - microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Penicillin polymerase chain reaction Polymerase Chain Reaction - methods repetitive sequences Staphylococcal Food Poisoning - microbiology Staphylococcal Infections - microbiology Staphylococcus aureus Staphylococcus aureus - classification Staphylococcus aureus - genetics superantigens Superantigens - classification Superantigens - genetics Toxins
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Beschreibung
Zusammenfassung:	We describe a comprehensive detection system for 18 kinds of classical and newly described staphylococcal superantigenic toxin genes using four sets of multiplex PCR. Superantigenic toxin genotyping of Staphylococcus aureus for 69 food poisoning isolates and 97 healthy human nasal swab isolates revealed 32 superantigenic toxin genotypes and showed that many S. aureus isolates harbored multiple toxin genes. Analysis of the relationship between toxin genotypes and toxin genes encoding profiles of mobile genetic elements suggests its possible role in determining superantigenic toxin genotypes in S. aureus as combinations of toxin gene-encoding mobile genetic elements.
ISSN:	0378-1097 1574-6968
DOI:	10.1016/j.femsle.2005.04.007