A Soy-Based Alternative to Traditional Bacterial Nutrient Media

Media recipes and their corresponding designations include: * SF= 3% soy flour * SP = 3% soy flour + 2% potato flour * SR = 3% soy flour + 2% rice bran * SY = 3% soy flour + 2% yeast extract * SPY = 3% soy flour + 2% potato flour + 2% yeast extract * SRY = 3% soy flour + 2% rice bran + 2% yeast extract * NB = nutrient broth (made according to manufacturer's instructions) * NBY = nutrient broth + 2% yeast extract * YE = 2% yeast extract * 2SF = 2% soy flour * MSY (modified soy media) = 2% soy flour + 0.5% yeast extract Qualitative Growth Analyses Fresh, single colonies (< 40 hours old) of all microbes tested were streaked with a wire loop onto agar plates with various ingredients.