Alive but Confused: Heterogeneity of CD11c+ MHC Class II+ Cells in GM-CSF Mouse Bone Marrow Cultures

Main Text Lutz et al take issue with our recent article documenting the heterogeneity of CD11c+MHC II (major histocompatibility complex class II)+ cells in cultures of mouse bone marrow (BM) with GM-CSF (Helft et al., 2015). [...]we believe that most researchers who use this culture system have considered the CD11c+MHC II+ fraction of those cultures to represent DCs that differ only in maturational status. [...]our paper is not about the fact that murine BM cultures with GM-CSF contain Macs and DCs; rather, it is about the presence of those two cell types within the CD11c+MHC II+ population that has traditionally been thought to represent pure DCs.