Isolation of human anti-serum albumin Fab antibodies with an extended serum-half life

Highlights • Human anti-serum albumin Fab antibodies was isolated via phage display technology. • One of Fabs, SL335, is cross-reactive with serum albumins at both pH 6 and pH 7.4. • SL335 showed much longer serum half-life than a negative control human Fab in rats.

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Bibliographische Detailangaben
Veröffentlicht in:	Immunology letters 2016-01, Vol.169, p.33-40
Hauptverfasser:	Kang, Hyeon-Ju, Kim, Hye-Jin, Cha, Sang-Hoon
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology Animals Cell Surface Display Techniques Cross Reactions Dogs Fab Half-Life Haplorhini Humans Hydrogen-Ion Concentration Immunoglobulin Fab Fragments - administration & dosage Immunoglobulin Fab Fragments - isolation & purification Immunoglobulin Fab Fragments - pharmacology Immunotherapy Mice Periplasmic expression Phage display Protein Binding Rats Rats, Sprague-Dawley Serum albumin Serum Albumin - immunology Serum Albumin - metabolism Serum half-life
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Beschreibung
Zusammenfassung:	Highlights • Human anti-serum albumin Fab antibodies was isolated via phage display technology. • One of Fabs, SL335, is cross-reactive with serum albumins at both pH 6 and pH 7.4. • SL335 showed much longer serum half-life than a negative control human Fab in rats.
ISSN:	0165-2478 1879-0542
DOI:	10.1016/j.imlet.2015.11.013