Complementation analysis reveals a potential role of human ARV1 in GPI anchor biosynthesis

Complementation analysis reveals a potential role of human ARV1 in GPI anchor biosynthesis

ARV1 is involved in regulating lipid homeostasis but also in the biosynthesis of glycosylphosphatidylinositol (GPI) in Saccharomyces cerevisiae. Here, we examined whether human ARV1 can complement the role of yeast ARV1 in GPI biosynthesis. Overexpression of human ARV1 could rescue the phenotypes as...

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Veröffentlicht in:Yeast (Chichester, England) England), 2016-02, Vol.33 (2), p.37-42
Hauptverfasser: Ikeda, Atsuko, Kajiwara, Kentaro, Iwamoto, Kunihiko, Makino, Asami, Kobayashi, Toshihide, Mizuta, Keiko, Funato, Kouichi
Format: Artikel
Sprache:eng
Schlagworte:
arv1Δ mutant
Carrier Proteins - genetics
Carrier Proteins - metabolism
complementation
Gene Expression
Genetic Complementation Test
Glycosylphosphatidylinositols - biosynthesis
GPI anchor
Homeostasis
human ARV1
Humans
Lipid Metabolism
Membrane Proteins - genetics
Membrane Proteins - metabolism
Mutation
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
yeast
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Zusammenfassung:ARV1 is involved in regulating lipid homeostasis but also in the biosynthesis of glycosylphosphatidylinositol (GPI) in Saccharomyces cerevisiae. Here, we examined whether human ARV1 can complement the role of yeast ARV1 in GPI biosynthesis. Overexpression of human ARV1 could rescue the phenotypes associated with GPI anchor synthesis defect in the yeast arv1Δ mutant. The results suggest that Arv1 function in GPI biosynthesis may be conserved in all eukaryotes, from yeast to humans. Copyright © 2015 John Wiley & Sons, Ltd.
ISSN:0749-503X
1097-0061
DOI:10.1002/yea.3138