Circular permutation of chicken interleukin-1 beta enhances its thermostability

Interleukin-1β is a cytokine critically involved in immune and inflammatory responses. To extend its use as a component of avian vaccines, a circularly permuted chicken interleukin-1β was synthesized that maintains its activity after pre-incubation at high temperatures, unlike wild-type chicken inte...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2014-04, Vol.50 (32), p.4248-4250
Hauptverfasser:	Chen, Wen-Ting, Chen, Ting, Cheng, Chao-Sheng, Huang, Wen-Yang, Wang, Xinquan, Yin, Hsien-Sheng
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chickens Chickens - immunology Chickens - metabolism Circular Dichroism Circularity Cytokines Fluorescence Hot Temperature Interleukin Interleukin-1beta - chemistry Interleukin-1beta - genetics Interleukin-1beta - metabolism Mutation - genetics Permutations Protein Stability Receptors, Interleukin-1 - metabolism Surface Plasmon Resonance Thermal stability Vaccines
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Beschreibung
Zusammenfassung:	Interleukin-1β is a cytokine critically involved in immune and inflammatory responses. To extend its use as a component of avian vaccines, a circularly permuted chicken interleukin-1β was synthesized that maintains its activity after pre-incubation at high temperatures, unlike wild-type chicken interleukin-1β, which is irreversibly inactivated at high temperatures.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c3cc48313d