Probing the Catalytic Promiscuity of a Regio- and Stereospecific C-Glycosyltransferase from Mangifera indica

The catalytic promiscuity of the novel benzophenone C‐glycosyltransferase, MiCGT, which is involved in the biosynthesis of mangiferin from Mangifera indica, was explored. MiCGT exhibited a robust capability to regio‐ and stereospecific C‐glycosylation of 35 structurally diverse druglike scaffolds and simple phenolics with UDP‐glucose, and also formed O‐ and N‐glycosides. Moreover, MiCGT was able to generate C‐xylosides with UDP‐xylose. The OGT‐reversibility of MiCGT was also exploited to generate C‐glucosides with simple sugar donor. Three aryl‐C‐glycosides exhibited potent SGLT2 inhibitory activities with IC50 values of 2.6×, 7.6×, and 7.6×10−7 M, respectively. These findings demonstrate for the first time the significant potential of an enzymatic approach to diversification through C‐glycosidation of bioactive natural and unnatural products in drug discovery. C‐Glykodiversifizierung: MiCGT, die erste Benzophenon‐C‐Glykosyltransferase (CGT) aus Mangifera indica, weist robuste regio‐ und stereospezifische C‐Glykosylierungsaktivität für 35 strukturell diverse Akzeptoren in Reaktionen mit UDP‐Glukose oder ‐Xylose auf. Das Aryl‐C‐Glykosid 1 zeigt eine potente antidiabetische Aktivität gegen SGLT2.