Microbial Toxicity of a Type of Carbon Dots to Escherichia coli

Carbon dots (Cdots), as a class of novel photoluminescence nanoprobes, has attracted tremendous interest for its broad application in recent years. Thus, the toxicity and behavior of Cdots in biological systems become important fundamental problems that require significant attention. In this study, Cdots with diameters of 5 nm are produced using mixed-acid treatment. The Cdots exhibit strong yellow fluorescence under UV irradiation and shifted emission peaks as the excitation wavelength is changed. Gram-negative bacteria Escherichia coli ( E. coli ) are applied as testing model to study the biological effect of Cdots on the cell growth by microcalorimetric, spectroscopic, and microscopic investigation. The introducing of Cdots caused a gradual increase of the maximum heat power ( P peak ) and the total heat produced ( Q total ) at low concentrations (0.0–5.00 mg/L). The metabolism rate constant ( k ) and half inhibitory concentration (IC 50 ) were calculated from the microcalorimetric data. The results indicated that Cdots had a concentration-dependent effect on the growth of E. coli . For confirmation, the growth curves and colony-forming units at different concentration of Cdots were studied. The morphology of E. coli in the absence and presence of Cdots was determined by scanning electron microscopy (SEM). The results of these studies were in agreement well with the analysis explored from microcalorimetry.