BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs

Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50. We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionari...

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Veröffentlicht in:Bioinformatics 2015-10, Vol.31 (19), p.3210-3212
Hauptverfasser: Simão, Felipe A, Waterhouse, Robert M, Ioannidis, Panagiotis, Kriventseva, Evgenia V, Zdobnov, Evgeny M
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Sprache:eng
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Zusammenfassung:Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50. We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO. Software implemented in Python and datasets available for download from http://busco.ezlab.org. evgeny.zdobnov@unige.ch Supplementary data are available at Bioinformatics online.
ISSN:1367-4803
1367-4811
1460-2059
DOI:10.1093/bioinformatics/btv351