BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs
Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50. We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionari...
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Veröffentlicht in: | Bioinformatics 2015-10, Vol.31 (19), p.3210-3212 |
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Sprache: | eng |
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Zusammenfassung: | Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50.
We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO.
Software implemented in Python and datasets available for download from http://busco.ezlab.org.
evgeny.zdobnov@unige.ch
Supplementary data are available at Bioinformatics online. |
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ISSN: | 1367-4803 1367-4811 1460-2059 |
DOI: | 10.1093/bioinformatics/btv351 |