Morphology Controlled Poly(aminophenylboronic acid) Nanostructures as Smart Substrates for Enhanced Capture and Release of Circulating Tumor Cells

A strategy is proposed to achieve an enhanced capture efficiency of and low damage to human leukemic lymphoblasts (CCRF‐CEM) by the synergistic effect of topographical interactions and phenylboronic acid functional groups on nanostructures. To realize this purpose, a simple and template free method to synthesize boronic acid derivative polyaniline bioinspired nanostructures with controlled morphology is established. Different nanostructured morphologies such as nanotexture, nanofibers, nanoparticles, microsphere, and 3D porous network have been prepared by controlling the nucleation and growth rate for polymerization. The phenylboronic acid functional groups on the surface of the nanostructures during poly­merization are used as artificial lectins to reversibly capture and release circulating tumor cells (CTCs) with little damage to the cells. The method presented here is simple, rapid, and highly efficient for CTC capture and release with low cost in materials and instruments. A synergistic effect of topographical interactions and surface chemistry of phenylboronic acid functional groups on nanostructures is proposed to achieve an enhanced capture efficiency of human leukemic lymphoblasts with reduced damage. Boronic acid derivative polyaniline bioinspired nanostructures with controlled morphology are prepared. The phenylboronic acid is used to reversibly capture and release circulating tumor cells.