Iterative operations on microdroplets and continuous monitoring of processes within them; determination of solubility diagrams of proteins

We demonstrate a technique for controlling the content of multiple microdroplets in time. We use this system to rapidly and quantiatively determine the solubility diagrams of two model proteins (lysozyme and ribonuclease A).

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Bibliographische Detailangaben
Veröffentlicht in:	Lab on a chip 2012, Vol.12 (20), p.4022-4025
Hauptverfasser:	Dolega, Monika E, Jakiela, Slawomir, Razew, Michal, Rakszewska, Agata, Cybulski, Olgierd, Garstecki, Piotr
Format:	Artikel
Sprache:	eng
Schlagworte:	Chips Iterative methods Lysozyme Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Monitoring Muramidase - chemistry Proteins Ribonuclease A Ribonuclease, Pancreatic - chemistry Solubility
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Beschreibung
Zusammenfassung:	We demonstrate a technique for controlling the content of multiple microdroplets in time. We use this system to rapidly and quantiatively determine the solubility diagrams of two model proteins (lysozyme and ribonuclease A).
ISSN:	1473-0197 1473-0189
DOI:	10.1039/c2lc40174f