Soot and house dust mite allergen cause eosinophilic laryngitis in an animal model

Objectives/Hypothesis Chronic laryngitis (CL) is common and costly. One of the most common causes of CL is thought to be laryngopharyngeal reflux, although a significant percentage of individuals fail to get better with acid suppressive therapy. The role of other potential causes of CL such as allergy and environmental pollution has not been thoroughly investigated. Purpose To evaluate the association between iron soot, house dust mite allergen (HDMA), and CL in an established animal model. Methods Twenty‐four guinea pigs were separated into four 6‐week exposure groups: 1) saline (allergen control) + filtered air (pollution control); 2) HDMA (Dermatophygoides farinae) + filtered air; 3) saline + combustion particulates; or 4) HDMA + combustion particulates. The primary outcome measure was mean eosinophil profile (MEP) in glottic, subglottic, and trachea epithelium and submucosa. Results The combination of iron soot and HDMA caused eosinophilia (elevated MEP) in the glottic (P