Fatty acid analysis of triacylglycerols: Preparation of fatty acid methyl esters for gas chromatography
A method to prepare fatty acid methyl esters was developed for fatty acid analysis of triacylglycerols by gas chromatography (GC). Triacylglycerols were mixed with methanolic CH3ONa in hexane containing a mid-polar solvent for 10 s at room temperature. Under these conditions, trioleoylglycerol was c...
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| Veröffentlicht in: | Analytical biochemistry 2016-02, Vol.495, p.6-8 |
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| container_title | Analytical biochemistry |
| container_volume | 495 |
| creator | Ichihara, Ken'ichi Kohsaka, Chihiro Tomari, Naohiro Kiyono, Tamami Wada, Jun Hirooka, Kiyoo Yamamoto, Yoshihiro |
| description | A method to prepare fatty acid methyl esters was developed for fatty acid analysis of triacylglycerols by gas chromatography (GC). Triacylglycerols were mixed with methanolic CH3ONa in hexane containing a mid-polar solvent for 10 s at room temperature. Under these conditions, trioleoylglycerol was converted to methyl oleate with an average yield of 99.3%. This procedure gave reliable and reproducible data on fatty acid compositions determined by GC. |
| doi_str_mv | 10.1016/j.ab.2015.11.009 |
| format | Article |
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Triacylglycerols were mixed with methanolic CH3ONa in hexane containing a mid-polar solvent for 10 s at room temperature. Under these conditions, trioleoylglycerol was converted to methyl oleate with an average yield of 99.3%. This procedure gave reliable and reproducible data on fatty acid compositions determined by GC.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/j.ab.2015.11.009</identifier><identifier>PMID: 26656926</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Chromatography, Gas ; Esters - chemistry ; Fatty acid analysis ; Fatty acid methyl ester ; Fatty Acids - analysis ; Gas chromatography ; Hexanes - chemistry ; Methanol - chemistry ; Oleic Acids - analysis ; Oleic Acids - metabolism ; Temperature ; Triacylglycerol ; Triglycerides - chemistry</subject><ispartof>Analytical biochemistry, 2016-02, Vol.495, p.6-8</ispartof><rights>2015 Elsevier Inc.</rights><rights>Copyright © 2015 Elsevier Inc. 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Triacylglycerols were mixed with methanolic CH3ONa in hexane containing a mid-polar solvent for 10 s at room temperature. Under these conditions, trioleoylglycerol was converted to methyl oleate with an average yield of 99.3%. This procedure gave reliable and reproducible data on fatty acid compositions determined by GC.</description><subject>Chromatography, Gas</subject><subject>Esters - chemistry</subject><subject>Fatty acid analysis</subject><subject>Fatty acid methyl ester</subject><subject>Fatty Acids - analysis</subject><subject>Gas chromatography</subject><subject>Hexanes - chemistry</subject><subject>Methanol - chemistry</subject><subject>Oleic Acids - analysis</subject><subject>Oleic Acids - metabolism</subject><subject>Temperature</subject><subject>Triacylglycerol</subject><subject>Triglycerides - chemistry</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kDFv2zAQhYmgReKk3TsVHLtIPZIiZWYrgiQNEKAd2pk4UiebhmS6pFxA_z4ynLZTp1u-9_DuY-yDgFqAMJ93NfpagtC1EDWAvWArAdZUoMC-YSsAUJU0tr1i16XsAIRotLlkV9IYbaw0K7Z5wGmaOYbYcdzjMJdYeOr5lCOGedgMc6CchnLLv2c6YMYppv0J6P_lRpq288CpTJQL71PmGyw8bHMacUqbjIft_I697XEo9P713rCfD_c_7r5Wz98en-6-PFdBaZgqgoCNxJasJ28E9ZZUC6i6tbfCBCLtlfJK-rZZSwjr0Bqvm7bBoHUrlVY37NO595DTr-MyyY2xBBoG3FM6FidaA2sjlTULCmc05FRKpt4dchwxz06AO-l1O4fenfQ6Idyid4l8fG0_-pG6v4E_Phfg9gzQ8uPvSNmVEGkfqIuZwuS6FP_f_gLurIth</recordid><startdate>20160215</startdate><enddate>20160215</enddate><creator>Ichihara, Ken'ichi</creator><creator>Kohsaka, Chihiro</creator><creator>Tomari, Naohiro</creator><creator>Kiyono, Tamami</creator><creator>Wada, Jun</creator><creator>Hirooka, Kiyoo</creator><creator>Yamamoto, Yoshihiro</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160215</creationdate><title>Fatty acid analysis of triacylglycerols: Preparation of fatty acid methyl esters for gas chromatography</title><author>Ichihara, Ken'ichi ; Kohsaka, Chihiro ; Tomari, Naohiro ; Kiyono, Tamami ; Wada, Jun ; Hirooka, Kiyoo ; Yamamoto, Yoshihiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-e0ca42a7e9beb61ef9e370a3d8b916cee5b33b32b74820c8c76b5474ac5572353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Chromatography, Gas</topic><topic>Esters - chemistry</topic><topic>Fatty acid analysis</topic><topic>Fatty acid methyl ester</topic><topic>Fatty Acids - analysis</topic><topic>Gas chromatography</topic><topic>Hexanes - chemistry</topic><topic>Methanol - chemistry</topic><topic>Oleic Acids - analysis</topic><topic>Oleic Acids - metabolism</topic><topic>Temperature</topic><topic>Triacylglycerol</topic><topic>Triglycerides - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ichihara, Ken'ichi</creatorcontrib><creatorcontrib>Kohsaka, Chihiro</creatorcontrib><creatorcontrib>Tomari, Naohiro</creatorcontrib><creatorcontrib>Kiyono, Tamami</creatorcontrib><creatorcontrib>Wada, Jun</creatorcontrib><creatorcontrib>Hirooka, Kiyoo</creatorcontrib><creatorcontrib>Yamamoto, Yoshihiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ichihara, Ken'ichi</au><au>Kohsaka, Chihiro</au><au>Tomari, Naohiro</au><au>Kiyono, Tamami</au><au>Wada, Jun</au><au>Hirooka, Kiyoo</au><au>Yamamoto, Yoshihiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fatty acid analysis of triacylglycerols: Preparation of fatty acid methyl esters for gas chromatography</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2016-02-15</date><risdate>2016</risdate><volume>495</volume><spage>6</spage><epage>8</epage><pages>6-8</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>A method to prepare fatty acid methyl esters was developed for fatty acid analysis of triacylglycerols by gas chromatography (GC). Triacylglycerols were mixed with methanolic CH3ONa in hexane containing a mid-polar solvent for 10 s at room temperature. Under these conditions, trioleoylglycerol was converted to methyl oleate with an average yield of 99.3%. This procedure gave reliable and reproducible data on fatty acid compositions determined by GC.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>26656926</pmid><doi>10.1016/j.ab.2015.11.009</doi><tpages>3</tpages></addata></record> |
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| ispartof | Analytical biochemistry, 2016-02, Vol.495, p.6-8 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Chromatography, Gas Esters - chemistry Fatty acid analysis Fatty acid methyl ester Fatty Acids - analysis Gas chromatography Hexanes - chemistry Methanol - chemistry Oleic Acids - analysis Oleic Acids - metabolism Temperature Triacylglycerol Triglycerides - chemistry |
| title | Fatty acid analysis of triacylglycerols: Preparation of fatty acid methyl esters for gas chromatography |
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