Fatty acid analysis of triacylglycerols: Preparation of fatty acid methyl esters for gas chromatography

A method to prepare fatty acid methyl esters was developed for fatty acid analysis of triacylglycerols by gas chromatography (GC). Triacylglycerols were mixed with methanolic CH3ONa in hexane containing a mid-polar solvent for 10 s at room temperature. Under these conditions, trioleoylglycerol was c...

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Veröffentlicht in:	Analytical biochemistry 2016-02, Vol.495, p.6-8
Hauptverfasser:	Ichihara, Ken'ichi, Kohsaka, Chihiro, Tomari, Naohiro, Kiyono, Tamami, Wada, Jun, Hirooka, Kiyoo, Yamamoto, Yoshihiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, Gas Esters - chemistry Fatty acid analysis Fatty acid methyl ester Fatty Acids - analysis Gas chromatography Hexanes - chemistry Methanol - chemistry Oleic Acids - analysis Oleic Acids - metabolism Temperature Triacylglycerol Triglycerides - chemistry
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Beschreibung
Zusammenfassung:	A method to prepare fatty acid methyl esters was developed for fatty acid analysis of triacylglycerols by gas chromatography (GC). Triacylglycerols were mixed with methanolic CH3ONa in hexane containing a mid-polar solvent for 10 s at room temperature. Under these conditions, trioleoylglycerol was converted to methyl oleate with an average yield of 99.3%. This procedure gave reliable and reproducible data on fatty acid compositions determined by GC.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/j.ab.2015.11.009