A Comparison of Apoptosis and Necrosis Induced by Hepatotoxins in HepG2 Cells

7H-Dibenzo[c,g]carbazole (DBC), an N-heterocyclic aromatic hydrocarbon, is cytotoxic and carcinogenic in rodent liver. While DBC leads to necrotic lesions in the liver, the induction of apoptosis by DBC has not been investigated. The focus of this study was to determine the degree to which apoptosis...

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Veröffentlicht in:	Toxicology and applied pharmacology 2000-05, Vol.164 (3), p.280-290
Hauptverfasser:	O'Brien, Travis, Babcock, George, Cornelius, James, Dingeldein, Mike, Talaska, Glenn, Warshawsky, David, Mitchell, Kent
Format:	Artikel
Sprache:	eng
Schlagworte:	7H-dibenzo[c,g]carbazole Adenosine Triphosphate - metabolism aflatoxin B1 Aflatoxin B1 - pharmacology Apoptosis ATP Biological and medical sciences Carbazoles - pharmacology Carcinogens - pharmacology cytotoxicity dibenzocarbazole DNA Damage - drug effects DNA Fragmentation General aspects. Methods Hepatoblastoma - pathology Humans In Situ Nick-End Labeling L-Lactate Dehydrogenase - secretion Liver Neoplasms - chemically induced Liver Neoplasms - pathology Medical sciences Microscopy, Electron N-heterocyclic aromatic hydrocarbons Necrosis Toxicology Tumor Cells, Cultured Tumor Suppressor Protein p53 - metabolism
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Zusammenfassung:	7H-Dibenzo[c,g]carbazole (DBC), an N-heterocyclic aromatic hydrocarbon, is cytotoxic and carcinogenic in rodent liver. While DBC leads to necrotic lesions in the liver, the induction of apoptosis by DBC has not been investigated. The focus of this study was to determine the degree to which apoptosis and necrosis contributed to DBC cytotoxicity in a human hepatoma cell line (HepG2). To determine if these effects were unique to DBC, the results were compared to another hepatotoxin, aflatoxin B1 (AFB1). DBC produced a distinct biphasic LDH release curve within 24 h of exposure. During the same time period lower concentrations of DBC (
ISSN:	0041-008X 1096-0333
DOI:	10.1006/taap.2000.8917