A simplified PCR-based method for detection of gray coat color allele in horse

A simplified PCR-based method for detection of gray coat color allele in horse

Coat color of gray horses is associated with a 4.6-kb duplication, which can be determined using PCR amplification of about 5-kb DNA fragment. In practice, this means that amplification might fail frequently. Therefore, a novel genetic screening method based on amplification of the 246 bp DNA fragme...

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Veröffentlicht in:Molecular and cellular probes 2012-12, Vol.26 (6), p.256-258
Hauptverfasser: Kavar, Tatjana, Čeh, Eva, Dovč, Peter
Format: Artikel
Sprache:eng
Schlagworte:
Alleles
Animals
Base Sequence
Duplication
Equus caballus
Gene Duplication
Genes, Dominant
Genetic Testing
Genotyping Techniques
Graying
Hair Color - genetics
Horses - genetics
Horses - physiology
Polymerase Chain Reaction
Qa-SNARE Proteins - genetics
Sequence Analysis, DNA
STX17
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Beschreibung
Zusammenfassung:Coat color of gray horses is associated with a 4.6-kb duplication, which can be determined using PCR amplification of about 5-kb DNA fragment. In practice, this means that amplification might fail frequently. Therefore, a novel genetic screening method based on amplification of the 246 bp DNA fragment has been developed.
ISSN:0890-8508
1096-1194
DOI:10.1016/j.mcp.2012.02.006