Ecdysteroid constituents of the mushroom Tapinella panuoides

Ecdysteroids, compounds structurally related to the insect moulting hormone ecdysone, were isolated from the mushroom Tapinella panuoides. Paxillosterone and two further new ergostane-type ecdysteroids, panuosterone and 25-hydroxypanuosterone, together with the already known cholestane-type ecdysteroids, 20-hydroxyecdysone, ponasterone A, malacosterone and turkesterone were identified by NMR, IR and mass spectrometric methods. The taxonomic value of ecdysteroids for chemosystematics in fungi and particularly in the genera Paxillus and Tapinella, is discussed.