Evaluation of DNA damage in Dicentrarchus labrax sperm following cryopreservation

Evaluation of DNA damage in Dicentrarchus labrax sperm following cryopreservation

In this paper, DNA laddering analysis and single-cell gel electrophoresis (SCGE) or Comet assay, were used to detect DNA damage in response to a cryopreservation process in sea bass spermatozoa. The results obtained demonstrate that the cryopreservation protocol used to cryopreserve the sea bass spe...

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Veröffentlicht in:Cryobiology 2003-12, Vol.47 (3), p.227-235
Hauptverfasser: Zilli, L., Schiavone, R., Zonno, V., Storelli, C., Vilella, S.
Format: Artikel
Sprache:eng
Schlagworte:
Animal aquaculture
Animal productions
Animals
Aquaculture
Bass
Biological and medical sciences
Comet Assay
Cryopreservation
Dicentrarchus labrax
DNA Damage
DNA Fragmentation
DNA integrity
Fish sperm
Fundamental and applied biological sciences. Psychology
Male
Marine
Pisciculture
SCGE
Semen Preservation - methods
Spermatozoa - pathology
Vertebrate aquaculture
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Zusammenfassung:In this paper, DNA laddering analysis and single-cell gel electrophoresis (SCGE) or Comet assay, were used to detect DNA damage in response to a cryopreservation process in sea bass spermatozoa. The results obtained demonstrate that the cryopreservation protocol used to cryopreserve the sea bass sperm cause significantly damage at DNA level. In fact, the degree of DNA damage in frozen-thawed sperm ( % DNA T =38.2±11.2 , M T=498.9±166.4, n=3) was different ( P
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2003.10.002