Use of the 2-chlorotrityl chloride resin for microwave-assisted solid phase peptide synthesis

ABSTRACT A fast and efficient microwave (MW)‐assisted solid‐phase peptide synthesis protocol using the 2‐chlorotrityl chloride resin and the Fmoc/tBu methodology, has been developed. The established protocol combines the advantages of MW irradiation and the acid labile 2‐chlorotrityl chloride resin. The effect of temperature during the MW irradiation, the degree of resin substitution during the coupling of the first amino acids and the rate of racemization for each amino acid were evaluated. The suggested solid phase methodology is applicable for orthogonal peptide synthesis and for the synthesis of cyclic peptides. © 2015 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 104: 506–514, 2015.