Surface immobilization of a protease through an inhibitor-derived affinity ligand: a bioactive surface with defensive properties against an inhibitor
The concept of enzyme immobilization via an inhibitor-derived peptide was developed. This method of immobilization was shown to be advantageous over physical adsorption and covalent bonding in retaining the enzymatic activity. Moreover, the surface-immobilized enzyme exhibited resistance against its...
Gespeichert in:
| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2015-09, Vol.51 (75), p.14263-14266 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 14266 |
|---|---|
| container_issue | 75 |
| container_start_page | 14263 |
| container_title | Chemical communications (Cambridge, England) |
| container_volume | 51 |
| creator | Tang, Zengchao Luan, Yafei Li, Dan Du, Hui Haddleton, David M Chen, Hong |
| description | The concept of enzyme immobilization via an inhibitor-derived peptide was developed. This method of immobilization was shown to be advantageous over physical adsorption and covalent bonding in retaining the enzymatic activity. Moreover, the surface-immobilized enzyme exhibited resistance against its inhibitor due to the occupation of an inhibitor binding site on the enzyme. |
| doi_str_mv | 10.1039/c5cc05652g |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1753527983</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1709715610</sourcerecordid><originalsourceid>FETCH-LOGICAL-c320t-84e2778ea0ea55c5b25222e37c089a6b1dc69637a73fd448b2563ed776d84d593</originalsourceid><addsrcrecordid>eNqNkc-K1EAQxhtR3HX04gNIH0WIdrrTf-JNgq7CggcVvIVKd2WmJEmP3R1lfQ_f14w7Ct6sSxX1ffWj4GPscS2e10K1L7z2Xmij5f4Ou6yVaSrduM93T7NuK6safcEe5PxFbFVrd59dSCNN44y8ZD8_rGkEj5zmOQ400Q8oFBceRw78mGJByMjLIcV1f-CwcFoONFCJqQqY6BsGDuNIC5UbPtEelvByOxwogi-byvMZ_53KgQccccmn9UY-YiqEmcMeaMnlH_ZDdm-EKeOjc9-xT29ef-zeVtfvr951r64rr6QolWtQWusQBILWXg9SSylRWS9cC2aogzetURasGkPTuE03CoO1Jrgm6Fbt2NNb7vbP1xVz6WfKHqcJFoxr7murlZa2deo_rKK1tTZbIjv27NbqU8w54dgfE82Qbvpa9KfE-k533e_ErjbzkzN3HWYMf61_IlK_ANYUk6c</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1709715610</pqid></control><display><type>article</type><title>Surface immobilization of a protease through an inhibitor-derived affinity ligand: a bioactive surface with defensive properties against an inhibitor</title><source>MEDLINE</source><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>Tang, Zengchao ; Luan, Yafei ; Li, Dan ; Du, Hui ; Haddleton, David M ; Chen, Hong</creator><creatorcontrib>Tang, Zengchao ; Luan, Yafei ; Li, Dan ; Du, Hui ; Haddleton, David M ; Chen, Hong</creatorcontrib><description>The concept of enzyme immobilization via an inhibitor-derived peptide was developed. This method of immobilization was shown to be advantageous over physical adsorption and covalent bonding in retaining the enzymatic activity. Moreover, the surface-immobilized enzyme exhibited resistance against its inhibitor due to the occupation of an inhibitor binding site on the enzyme.</description><identifier>ISSN: 1359-7345</identifier><identifier>EISSN: 1364-548X</identifier><identifier>DOI: 10.1039/c5cc05652g</identifier><identifier>PMID: 26264862</identifier><language>eng</language><publisher>England</publisher><subject>Affinity ; Binding Sites ; Bonding ; Covalence ; Enzymes ; Enzymes, Immobilized - chemistry ; Enzymes, Immobilized - metabolism ; Immobilization ; Inhibitors ; Ligands ; Models, Molecular ; Molecular Structure ; Occupation ; Plasminogen Inactivators - chemistry ; Structure-Activity Relationship ; Surface chemistry ; Surface Properties ; Tissue Plasminogen Activator - chemistry ; Tissue Plasminogen Activator - metabolism</subject><ispartof>Chemical communications (Cambridge, England), 2015-09, Vol.51 (75), p.14263-14266</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c320t-84e2778ea0ea55c5b25222e37c089a6b1dc69637a73fd448b2563ed776d84d593</citedby><cites>FETCH-LOGICAL-c320t-84e2778ea0ea55c5b25222e37c089a6b1dc69637a73fd448b2563ed776d84d593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26264862$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tang, Zengchao</creatorcontrib><creatorcontrib>Luan, Yafei</creatorcontrib><creatorcontrib>Li, Dan</creatorcontrib><creatorcontrib>Du, Hui</creatorcontrib><creatorcontrib>Haddleton, David M</creatorcontrib><creatorcontrib>Chen, Hong</creatorcontrib><title>Surface immobilization of a protease through an inhibitor-derived affinity ligand: a bioactive surface with defensive properties against an inhibitor</title><title>Chemical communications (Cambridge, England)</title><addtitle>Chem Commun (Camb)</addtitle><description>The concept of enzyme immobilization via an inhibitor-derived peptide was developed. This method of immobilization was shown to be advantageous over physical adsorption and covalent bonding in retaining the enzymatic activity. Moreover, the surface-immobilized enzyme exhibited resistance against its inhibitor due to the occupation of an inhibitor binding site on the enzyme.</description><subject>Affinity</subject><subject>Binding Sites</subject><subject>Bonding</subject><subject>Covalence</subject><subject>Enzymes</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Enzymes, Immobilized - metabolism</subject><subject>Immobilization</subject><subject>Inhibitors</subject><subject>Ligands</subject><subject>Models, Molecular</subject><subject>Molecular Structure</subject><subject>Occupation</subject><subject>Plasminogen Inactivators - chemistry</subject><subject>Structure-Activity Relationship</subject><subject>Surface chemistry</subject><subject>Surface Properties</subject><subject>Tissue Plasminogen Activator - chemistry</subject><subject>Tissue Plasminogen Activator - metabolism</subject><issn>1359-7345</issn><issn>1364-548X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc-K1EAQxhtR3HX04gNIH0WIdrrTf-JNgq7CggcVvIVKd2WmJEmP3R1lfQ_f14w7Ct6sSxX1ffWj4GPscS2e10K1L7z2Xmij5f4Ou6yVaSrduM93T7NuK6safcEe5PxFbFVrd59dSCNN44y8ZD8_rGkEj5zmOQ400Q8oFBceRw78mGJByMjLIcV1f-CwcFoONFCJqQqY6BsGDuNIC5UbPtEelvByOxwogi-byvMZ_53KgQccccmn9UY-YiqEmcMeaMnlH_ZDdm-EKeOjc9-xT29ef-zeVtfvr951r64rr6QolWtQWusQBILWXg9SSylRWS9cC2aogzetURasGkPTuE03CoO1Jrgm6Fbt2NNb7vbP1xVz6WfKHqcJFoxr7murlZa2deo_rKK1tTZbIjv27NbqU8w54dgfE82Qbvpa9KfE-k533e_ErjbzkzN3HWYMf61_IlK_ANYUk6c</recordid><startdate>20150928</startdate><enddate>20150928</enddate><creator>Tang, Zengchao</creator><creator>Luan, Yafei</creator><creator>Li, Dan</creator><creator>Du, Hui</creator><creator>Haddleton, David M</creator><creator>Chen, Hong</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20150928</creationdate><title>Surface immobilization of a protease through an inhibitor-derived affinity ligand: a bioactive surface with defensive properties against an inhibitor</title><author>Tang, Zengchao ; Luan, Yafei ; Li, Dan ; Du, Hui ; Haddleton, David M ; Chen, Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c320t-84e2778ea0ea55c5b25222e37c089a6b1dc69637a73fd448b2563ed776d84d593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Affinity</topic><topic>Binding Sites</topic><topic>Bonding</topic><topic>Covalence</topic><topic>Enzymes</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Enzymes, Immobilized - metabolism</topic><topic>Immobilization</topic><topic>Inhibitors</topic><topic>Ligands</topic><topic>Models, Molecular</topic><topic>Molecular Structure</topic><topic>Occupation</topic><topic>Plasminogen Inactivators - chemistry</topic><topic>Structure-Activity Relationship</topic><topic>Surface chemistry</topic><topic>Surface Properties</topic><topic>Tissue Plasminogen Activator - chemistry</topic><topic>Tissue Plasminogen Activator - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tang, Zengchao</creatorcontrib><creatorcontrib>Luan, Yafei</creatorcontrib><creatorcontrib>Li, Dan</creatorcontrib><creatorcontrib>Du, Hui</creatorcontrib><creatorcontrib>Haddleton, David M</creatorcontrib><creatorcontrib>Chen, Hong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Chemical communications (Cambridge, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tang, Zengchao</au><au>Luan, Yafei</au><au>Li, Dan</au><au>Du, Hui</au><au>Haddleton, David M</au><au>Chen, Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surface immobilization of a protease through an inhibitor-derived affinity ligand: a bioactive surface with defensive properties against an inhibitor</atitle><jtitle>Chemical communications (Cambridge, England)</jtitle><addtitle>Chem Commun (Camb)</addtitle><date>2015-09-28</date><risdate>2015</risdate><volume>51</volume><issue>75</issue><spage>14263</spage><epage>14266</epage><pages>14263-14266</pages><issn>1359-7345</issn><eissn>1364-548X</eissn><abstract>The concept of enzyme immobilization via an inhibitor-derived peptide was developed. This method of immobilization was shown to be advantageous over physical adsorption and covalent bonding in retaining the enzymatic activity. Moreover, the surface-immobilized enzyme exhibited resistance against its inhibitor due to the occupation of an inhibitor binding site on the enzyme.</abstract><cop>England</cop><pmid>26264862</pmid><doi>10.1039/c5cc05652g</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1359-7345 |
| ispartof | Chemical communications (Cambridge, England), 2015-09, Vol.51 (75), p.14263-14266 |
| issn | 1359-7345 1364-548X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1753527983 |
| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Affinity Binding Sites Bonding Covalence Enzymes Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Immobilization Inhibitors Ligands Models, Molecular Molecular Structure Occupation Plasminogen Inactivators - chemistry Structure-Activity Relationship Surface chemistry Surface Properties Tissue Plasminogen Activator - chemistry Tissue Plasminogen Activator - metabolism |
| title | Surface immobilization of a protease through an inhibitor-derived affinity ligand: a bioactive surface with defensive properties against an inhibitor |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T20%3A54%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Surface%20immobilization%20of%20a%20protease%20through%20an%20inhibitor-derived%20affinity%20ligand:%20a%20bioactive%20surface%20with%20defensive%20properties%20against%20an%20inhibitor&rft.jtitle=Chemical%20communications%20(Cambridge,%20England)&rft.au=Tang,%20Zengchao&rft.date=2015-09-28&rft.volume=51&rft.issue=75&rft.spage=14263&rft.epage=14266&rft.pages=14263-14266&rft.issn=1359-7345&rft.eissn=1364-548X&rft_id=info:doi/10.1039/c5cc05652g&rft_dat=%3Cproquest_cross%3E1709715610%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1709715610&rft_id=info:pmid/26264862&rfr_iscdi=true |