Surface immobilization of a protease through an inhibitor-derived affinity ligand: a bioactive surface with defensive properties against an inhibitor

Surface immobilization of a protease through an inhibitor-derived affinity ligand: a bioactive surface with defensive properties against an inhibitor

The concept of enzyme immobilization via an inhibitor-derived peptide was developed. This method of immobilization was shown to be advantageous over physical adsorption and covalent bonding in retaining the enzymatic activity. Moreover, the surface-immobilized enzyme exhibited resistance against its...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2015-09, Vol.51 (75), p.14263-14266
Hauptverfasser: Tang, Zengchao, Luan, Yafei, Li, Dan, Du, Hui, Haddleton, David M, Chen, Hong
Format: Artikel
Sprache:eng
Schlagworte:
Affinity
Binding Sites
Bonding
Covalence
Enzymes
Enzymes, Immobilized - chemistry
Enzymes, Immobilized - metabolism
Immobilization
Inhibitors
Ligands
Models, Molecular
Molecular Structure
Occupation
Plasminogen Inactivators - chemistry
Structure-Activity Relationship
Surface chemistry
Surface Properties
Tissue Plasminogen Activator - chemistry
Tissue Plasminogen Activator - metabolism
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Beschreibung
Zusammenfassung:The concept of enzyme immobilization via an inhibitor-derived peptide was developed. This method of immobilization was shown to be advantageous over physical adsorption and covalent bonding in retaining the enzymatic activity. Moreover, the surface-immobilized enzyme exhibited resistance against its inhibitor due to the occupation of an inhibitor binding site on the enzyme.
ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc05652g