Measurement of homoarginine in human and mouse plasma by LC–MS/MS and ELISA: a comparison and a biological application
Homoarginine (hArg) is a non-essential amino acid that was identified as a risk marker for cardiovascular disease. Several analytical methods have been described for the quantification of hArg in biological samples. The aim of this study was to compare a liquid chromatography–tandem mass spectrometr...
Gespeichert in:
Veröffentlicht in: | Amino acids 2015-09, Vol.47 (9), p.2015-2022 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Homoarginine (hArg) is a non-essential amino acid that was identified as a risk marker for cardiovascular disease. Several analytical methods have been described for the quantification of hArg in biological samples. The aim of this study was to compare a liquid chromatography–tandem mass spectrometric (LC–MS/MS) approach with a commercially available enzyme-linked immunosorbent assay (ELISA). Determination of hArg concentrations in ELISA calibration standards measured by both methods revealed a correlation coefficient r ² of 0.99, for LC–MS/MS calibrators r ² was 0.997. However, linear regression analysis between the two assays for hArg concentrations in human plasma samples revealed a correlation coefficient r ² of 0.78. Plasma concentrations obtained from LC–MS/MS are on average 29 % higher than those by ELISA. We investigated the hArg-isobaric N ᵋ-trimethyllysine as potential source for the higher observed values, but evaluation of mass spectra indicated that N ᵋ-trimethyllysine did not interfere with hArg quantification in our LC–MS/MS method. Both quantification methods were applied to measure hArg in (1) a case–control study of acute coronary syndrome and (2) L-arginine:glycine amidinotransferase-deficient mice. Our LC–MS/MS and the commercially available ELISA assay are suitable for hArg measurement in human and mouse plasma, but different reference values for each method need to be considered. |
---|---|
ISSN: | 0939-4451 1438-2199 |
DOI: | 10.1007/s00726-015-2037-7 |