Tigriopus fulvus: The interlaboratory comparison of the acute toxicity test

The paper reports the results of an interlaboratory comparison involving 11 laboratories, with the objectives of apply and validate a new standardized ecotoxicological method on marine crustacean Tigriopus fulvus. Copper was chosen as reference toxicant as indicated in the official method. The results of two independent tests performed by all the participants, demonstrated that the new method is simple, fast and easy to learn. This is confirmed even by the values of z-score index calculated for each laboratory and the relative coefficient of variation (CV) which are 6.32% after 24h, 6.56 after 48h and 35.3% after 96h, mentioned in the ISO standards for the precision of interlaboratory assays. Therefore its use could be recommended in environmental studies and monitoring. •.The study demonstrated the efficiency of UNICHIM 2396:2014 with Tigriopus fulvus•.The variability was lower to previous interlaboratory exercises with Crustacea•R-values and Z-score confirmed the validity of our interlaboratory comparison.•The study verified that UNICHIM 2396:2014 is an easy-to-apply and reliable method.•The protocol is advisable for toxicology researches and environmental monitoring.