Enhancing cellular immunogenicity of MVA-vectored vaccines by utilizing the F11L endogenous promoter

Enhancing cellular immunogenicity of MVA-vectored vaccines by utilizing the F11L endogenous promoter

Highlights • This study presents the effect of stronger promoters on MVA immunogenicity. • F11 promoter (pF11) enhanced in vivo transgene expression and immunogenicity. • pF11 proved stronger than mH5 and p7.5 promoters. • pF11 was tested with a reporter transgene as well as candidate vaccine antige...

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Veröffentlicht in:Vaccine 2016-01, Vol.34 (1), p.49-55
Hauptverfasser: Alharbi, Naif Khalaf, Spencer, Alexandra J, Salman, Ahmed M, Tully, Claire M, Chinnakannan, Senthil k, Lambe, Teresa, Yamaguchi, Yuko, Morris, Susan J, Orubu, Toritse, Draper, Simon J, Hill, Adrian V.S, Gilbert, Sarah C
Format: Artikel
Sprache:eng
Schlagworte:
Allergy and Immunology
Animals
Antigens
Biomedical research
Clinical trials
Drug Carriers
Ebola virus
Ebolavirus
Enzyme-Linked Immunospot Assay
Female
Gene Expression
Genetic Vectors
Genomes
Immunization
Immunogenicity
Influenza Vaccines - genetics
Influenza Vaccines - immunology
Interferon-gamma - metabolism
Kinases
Leukocytes, Mononuclear - immunology
Lymphocytes
Malaria
Malaria Vaccines - genetics
Malaria Vaccines - immunology
Medical research
Mice, Inbred BALB C
MVA
Mycobacterium
Promoter
Promoter Regions, Genetic
Proteins
Rodents
Studies
Transgene expression
Transgenes
Treatment Outcome
Vaccine development
Vaccines
Vaccines, Synthetic - genetics
Vaccines, Synthetic - immunology
Vaccinia virus
Vaccinia virus - genetics
Vector-borne diseases
Vectors (Biology)
Viral vector
Viruses
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Beschreibung
Zusammenfassung:Highlights • This study presents the effect of stronger promoters on MVA immunogenicity. • F11 promoter (pF11) enhanced in vivo transgene expression and immunogenicity. • pF11 proved stronger than mH5 and p7.5 promoters. • pF11 was tested with a reporter transgene as well as candidate vaccine antigens. • Mechanisms underlying the improved immunogenicity with pF11 were investigated.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2015.11.028