Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale

A panel of human flavin monooxygenases were heterologously expressed in E. coli to obtain ready-to-use biocatalysts for the in vitro preparation of human drug metabolites. Moclobemide-N-oxide (65 mg) was the first high-priced metabolite prepared with recombinant hFMO3 on the multi-milligram scale.

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Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2012-01, Vol.48 (48), p.6001-6003
Hauptverfasser:	Hanlon, Steven P, Camattari, Andrea, Abad, Sandra, Glieder, Anton, Kittelmann, Matthias, Lütz, Stephan, Wirz, Beat, Winkler, Margit
Format:	Artikel
Sprache:	eng
Schlagworte:	Enzymes Escherichia coli - chemistry Escherichia coli - metabolism Flavins - chemistry Flavins - metabolism Humans Mixed Function Oxygenases - chemistry Mixed Function Oxygenases - metabolism Moclobemide - chemical synthesis Moclobemide - chemistry
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Beschreibung
Zusammenfassung:	A panel of human flavin monooxygenases were heterologously expressed in E. coli to obtain ready-to-use biocatalysts for the in vitro preparation of human drug metabolites. Moclobemide-N-oxide (65 mg) was the first high-priced metabolite prepared with recombinant hFMO3 on the multi-milligram scale.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c2cc17878h