Distribution of HPA-genes and genotypes in the population of Russian unrelated donors

Race and populational distinctions in prevalence of HPA-genes (human platelet antigens) are known. Definition of frequency allele variants and genotypes HPA matters for immunogenetic and transfusion. The research problem - studying of distribution of HPA-genes among Russians. A method of research - polymerase chain reaction with allele-specific primers of firm "Protrans" for identification of twelve allele genes "a" and "b" of loci HPA-1,-2,-3,-4,-5 and -6. A material - DNA leukocytes of peripheral blood of 229 unrelated donors. Allele genes HPA- HPA-4A and HPA-6a met at all surveyed persons, HPA-1a - at 94,32%, HPA-2a -at 98,25%, HPA-3a - at 78,60%, HPA-3b - at 70,74% and HPA-5a - at 99,13 of Russians. Allele genes HPA-1b,-2b,-5b met approximately identical frequency - at 27,95%, 21,83% and 20,09% of individuals. Frequencies of genotypes are established: HPA-1a/a - 0,72, -1a/b - 0,22, -1b/b - 0,06; HPA-2a/a-0,78,-2a/b-0,20; -2b/b-0,017; HPA-3a/a-0,297,-3a/b-0,49,-3b/b-0,214; HPA-5a/a-0,8,-5a/b-0,19, 5b/b-0,009. Genotypes HPA-4b/b, HPA-6b/b have not been revealed. Gene frequencies have made 0,83 and 0,17 for HPA-1a and HPA-1b; 0,88 and 0,12 for HPA-2a and HPA-2b; 0,54 and 0,46 HPA-3a and HPA-3b; 1,0 and 0 for HPA-4a, 6a and-4b,-6b; 0,895 and 0,105 for HPA-5a and HPA-5b. The allele and genotypes frequencies of HPA at inhabitants of Russia are comparable to frequencies of genes in the East European population.