Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium

Multiple shoots of Curcuma longa were induced by culture of bud explants for 1 week in Murashige and Skoog (MS) liquid medium supplemented with 72.64 mu M thidiazuron (TDZ) prior to culture on MS gelled medium without growth regulator for 8 weeks. The regeneration rate was up to 11.4 plus or minus 1...

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Veröffentlicht in:	Plant cell, tissue and organ culture tissue and organ culture, 2005-03, Vol.80 (3), p.347-351
Hauptverfasser:	Prathanturarug, S, Soonthornchareonnon, N, Chuakul, W, Phaidee, Y, Saralamp, P
Format:	Artikel
Sprache:	eng
Schlagworte:	benzyladenine Biological and medical sciences Biotechnology buds culture media Curcuma longa dose response Eukaryotic cell cultures explants Fundamental and applied biological sciences. Psychology in vitro culture in vitro regeneration liquid medium medicinal plants Methods. Procedures. Technologies micropropagation Miscellaneous Murashige and Skoog medium Plant cells and fungal cells plant growth substances rapid methods thidiazuron turmeric
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Beschreibung
Zusammenfassung:	Multiple shoots of Curcuma longa were induced by culture of bud explants for 1 week in Murashige and Skoog (MS) liquid medium supplemented with 72.64 mu M thidiazuron (TDZ) prior to culture on MS gelled medium without growth regulator for 8 weeks. The regeneration rate was up to 11.4 plus or minus 1.7 shoots/explant. Rooting was spontaneous and the regenerated plants were successfully transferred to soil. This protocol can be an alternative for rapid micropropagation of C. longa used for phytomedicine raw material production.
ISSN:	0167-6857 1573-5044
DOI:	10.1007/s11240-004-1020-x