Isolation and characterization of microsatellite markers in the tetraploid birch, Betula pubescens ssp. tortuosa
The mountain birch, Betula pubescens ssp. tortuosa is a tetraploid tree species which forms the tree limit in northern Fennoscandia. We identified nine polymorphic microsatellite loci in order to characterize the genetic structure of populations at different elevations close to the treeline. The mic...
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| Veröffentlicht in: | Molecular ecology notes 2005-03, Vol.5 (1), p.96-98 |
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| creator | Truong, C Palme, A.E Felber, F Naciri-Graven, Y |
| description | The mountain birch, Betula pubescens ssp. tortuosa is a tetraploid tree species which forms the tree limit in northern Fennoscandia. We identified nine polymorphic microsatellite loci in order to characterize the genetic structure of populations at different elevations close to the treeline. The microsatellite loci were highly polymorphic, with 14–42 alleles per locus and an average expected heterozygosity of 0.73 ± 0.25 under random chromosome segregation and 0.68 ± 0.23 under random chromatid segregation. |
| doi_str_mv | 10.1111/j.1471-8286.2004.00848.x |
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We identified nine polymorphic microsatellite loci in order to characterize the genetic structure of populations at different elevations close to the treeline. The microsatellite loci were highly polymorphic, with 14–42 alleles per locus and an average expected heterozygosity of 0.73 ± 0.25 under random chromosome segregation and 0.68 ± 0.23 under random chromatid segregation.</description><identifier>ISSN: 1471-8278</identifier><identifier>EISSN: 1471-8286</identifier><identifier>DOI: 10.1111/j.1471-8286.2004.00848.x</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>alleles ; alleles per locus ; autopolyploidy ; Betula pubescens ; Betula pubescens subsp. tortuosa ; DNA primers ; genetic markers ; genetic structure ; heterozygosity ; loci ; microsatellite markers ; microsatellite repeats ; molecular sequence data ; mountain birch ; nonradioactive probes ; nucleotide sequences ; polymerase chain reaction ; polymorphic loci</subject><ispartof>Molecular ecology notes, 2005-03, Vol.5 (1), p.96-98</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4058-677e7f069ffe3d377f7d5d405851b0ad49e56a86dcabae3a2ca1eda64e84616f3</citedby><cites>FETCH-LOGICAL-c4058-677e7f069ffe3d377f7d5d405851b0ad49e56a86dcabae3a2ca1eda64e84616f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1471-8286.2004.00848.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1471-8286.2004.00848.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids></links><search><creatorcontrib>Truong, C</creatorcontrib><creatorcontrib>Palme, A.E</creatorcontrib><creatorcontrib>Felber, F</creatorcontrib><creatorcontrib>Naciri-Graven, Y</creatorcontrib><title>Isolation and characterization of microsatellite markers in the tetraploid birch, Betula pubescens ssp. tortuosa</title><title>Molecular ecology notes</title><description>The mountain birch, Betula pubescens ssp. tortuosa is a tetraploid tree species which forms the tree limit in northern Fennoscandia. We identified nine polymorphic microsatellite loci in order to characterize the genetic structure of populations at different elevations close to the treeline. The microsatellite loci were highly polymorphic, with 14–42 alleles per locus and an average expected heterozygosity of 0.73 ± 0.25 under random chromosome segregation and 0.68 ± 0.23 under random chromatid segregation.</description><subject>alleles</subject><subject>alleles per locus</subject><subject>autopolyploidy</subject><subject>Betula pubescens</subject><subject>Betula pubescens subsp. tortuosa</subject><subject>DNA primers</subject><subject>genetic markers</subject><subject>genetic structure</subject><subject>heterozygosity</subject><subject>loci</subject><subject>microsatellite markers</subject><subject>microsatellite repeats</subject><subject>molecular sequence data</subject><subject>mountain birch</subject><subject>nonradioactive probes</subject><subject>nucleotide sequences</subject><subject>polymerase chain reaction</subject><subject>polymorphic loci</subject><issn>1471-8278</issn><issn>1471-8286</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqNkMFu1DAQhiMEEqXwDPjEiQQ7TmxH4gKllEqlCC2FozWbjFkv3jjYjtjy9DgE7RlfPPL838jzFQVhtGL5vNpXrJGsVLUSVU1pU1GqGlUdHxRnp8bDUy3V4-JJjHtKmezq5qyYrqN3kKwfCYwD6XcQoE8Y7O_10RtysH3wERI6ZxOSA4QfGCKxI0k7JAlTgMl5O5CtDf3uJXmLaXZApnmLsccxkhiniiQf0pzHPC0eGXARn_27z4u795dfLj6UN5-uri_e3JR9Q1tVCilRGio6Y5APXEojh3ZYWi3bUhiaDlsBSgw9bAE51D0wHEA0qBrBhOHnxYt17hT8zxlj0gebv-McjOjnqJlsa96pOgfVGly2jAGNnoLNS95rRvWiWO_1Yk8vJvWiWP9VrI8Zfb2iv6zD-__m9MfL21xkvFxxGxMeT3j2q4XkstXfbq_0V7WR8nP3Tm9y_vmaN-A1fA826rtNTRmnjFJOJeV_AMLWnTU</recordid><startdate>200503</startdate><enddate>200503</enddate><creator>Truong, C</creator><creator>Palme, A.E</creator><creator>Felber, F</creator><creator>Naciri-Graven, Y</creator><general>Blackwell Science Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SN</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>200503</creationdate><title>Isolation and characterization of microsatellite markers in the tetraploid birch, Betula pubescens ssp. tortuosa</title><author>Truong, C ; Palme, A.E ; Felber, F ; Naciri-Graven, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4058-677e7f069ffe3d377f7d5d405851b0ad49e56a86dcabae3a2ca1eda64e84616f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>alleles</topic><topic>alleles per locus</topic><topic>autopolyploidy</topic><topic>Betula pubescens</topic><topic>Betula pubescens subsp. tortuosa</topic><topic>DNA primers</topic><topic>genetic markers</topic><topic>genetic structure</topic><topic>heterozygosity</topic><topic>loci</topic><topic>microsatellite markers</topic><topic>microsatellite repeats</topic><topic>molecular sequence data</topic><topic>mountain birch</topic><topic>nonradioactive probes</topic><topic>nucleotide sequences</topic><topic>polymerase chain reaction</topic><topic>polymorphic loci</topic><toplevel>online_resources</toplevel><creatorcontrib>Truong, C</creatorcontrib><creatorcontrib>Palme, A.E</creatorcontrib><creatorcontrib>Felber, F</creatorcontrib><creatorcontrib>Naciri-Graven, Y</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>CrossRef</collection><collection>Ecology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular ecology notes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Truong, C</au><au>Palme, A.E</au><au>Felber, F</au><au>Naciri-Graven, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of microsatellite markers in the tetraploid birch, Betula pubescens ssp. tortuosa</atitle><jtitle>Molecular ecology notes</jtitle><date>2005-03</date><risdate>2005</risdate><volume>5</volume><issue>1</issue><spage>96</spage><epage>98</epage><pages>96-98</pages><issn>1471-8278</issn><eissn>1471-8286</eissn><abstract>The mountain birch, Betula pubescens ssp. tortuosa is a tetraploid tree species which forms the tree limit in northern Fennoscandia. We identified nine polymorphic microsatellite loci in order to characterize the genetic structure of populations at different elevations close to the treeline. The microsatellite loci were highly polymorphic, with 14–42 alleles per locus and an average expected heterozygosity of 0.73 ± 0.25 under random chromosome segregation and 0.68 ± 0.23 under random chromatid segregation.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><doi>10.1111/j.1471-8286.2004.00848.x</doi><tpages>3</tpages></addata></record> |
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| ispartof | Molecular ecology notes, 2005-03, Vol.5 (1), p.96-98 |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | alleles alleles per locus autopolyploidy Betula pubescens Betula pubescens subsp. tortuosa DNA primers genetic markers genetic structure heterozygosity loci microsatellite markers microsatellite repeats molecular sequence data mountain birch nonradioactive probes nucleotide sequences polymerase chain reaction polymorphic loci |
| title | Isolation and characterization of microsatellite markers in the tetraploid birch, Betula pubescens ssp. tortuosa |
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