Isolation and characterization of microsatellite markers in the tetraploid birch, Betula pubescens ssp. tortuosa

The mountain birch, Betula pubescens ssp. tortuosa is a tetraploid tree species which forms the tree limit in northern Fennoscandia. We identified nine polymorphic microsatellite loci in order to characterize the genetic structure of populations at different elevations close to the treeline. The mic...

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Veröffentlicht in:	Molecular ecology notes 2005-03, Vol.5 (1), p.96-98
Hauptverfasser:	Truong, C, Palme, A.E, Felber, F, Naciri-Graven, Y
Format:	Artikel
Sprache:	eng
Schlagworte:	alleles alleles per locus autopolyploidy Betula pubescens Betula pubescens subsp. tortuosa DNA primers genetic markers genetic structure heterozygosity loci microsatellite markers microsatellite repeats molecular sequence data mountain birch nonradioactive probes nucleotide sequences polymerase chain reaction polymorphic loci
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Zusammenfassung:	The mountain birch, Betula pubescens ssp. tortuosa is a tetraploid tree species which forms the tree limit in northern Fennoscandia. We identified nine polymorphic microsatellite loci in order to characterize the genetic structure of populations at different elevations close to the treeline. The microsatellite loci were highly polymorphic, with 14–42 alleles per locus and an average expected heterozygosity of 0.73 ± 0.25 under random chromosome segregation and 0.68 ± 0.23 under random chromatid segregation.
ISSN:	1471-8278 1471-8286
DOI:	10.1111/j.1471-8286.2004.00848.x