Novel polymorphic microsatellite markers in section Caulorrhizae (Arachis, Fabaceae)

This work reports the characterization of 11 polymorphic microsatellite loci in section Caulorrhizae. The primer pairs were designed from Arachis pintoi and showed full transferability to Arachis repens species. These new markers were used to evaluate the genetic diversity in germplasm (accessions a...

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Veröffentlicht in:	Molecular ecology notes 2005-03, Vol.5 (1), p.77-79
Hauptverfasser:	Palmieri, D.A, Bechara, M.D, Curi, R.A, Gimenes, M.A, Lopes, C.R
Format:	Artikel
Sprache:	eng
Schlagworte:	alleles alleles per locus Arachis Arachis pintoi Arachis repens cultivars DNA primers Fabaceae genetic diversity genetic markers genetic variation germplasm germplasm evaluation heterozygosity loci microsatellite repeats microsatellites markers molecular sequence data nucleotide sequences plant genetic resources polymerase chain reaction polymorphic loci
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Zusammenfassung:	This work reports the characterization of 11 polymorphic microsatellite loci in section Caulorrhizae. The primer pairs were designed from Arachis pintoi and showed full transferability to Arachis repens species. These new markers were used to evaluate the genetic diversity in germplasm (accessions and cultivars) of section Caulorrhizae. This new set of markers detected greater gene diversity than morphological and molecular markers such as AFLP (amplified fragment length polymorphism) and RAPD (rapid analysis of polymorphic DNA) previously used in this germplasm.
ISSN:	1471-8278 1471-8286
DOI:	10.1111/j.1471-8286.2004.00838.x