Identification of biomarkers to measure HIV-specific mucosal and systemic CD8+ T-cell immunity using single cell Fluidigm 48.48 Dynamic arrays

Highlights • RANTES, MIP-1β, perforin, α4, β1 and β7: set of biomarkers to measure HIV-specific mucosal immunity. • Intensity of IL-13 inhibition can modulate the level of poly-functionality and CTL immunity. • According to the anatomical location immune markers expressed can be significantly differ...

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Veröffentlicht in:	Vaccine 2015-12, Vol.33 (51), p.7315-7327
Hauptverfasser:	Trivedi, Shubhanshi, Neeman, Teresa, Jackson, Ronald J, Ranasinghe, Roshanka, Jack, Cameron, Ranasinghe, Charani
Format:	Artikel
Sprache:	eng
Schlagworte:	Adjuvants, Immunologic - administration & dosage AIDS Vaccines - administration & dosage AIDS Vaccines - immunology Allergy and Immunology Animals Biomarkers Biomarkers - analysis CD8-Positive T-Lymphocytes - immunology Cell adhesion & migration Chemokines Female Flow cytometry Gene Expression Profiling Gene Products, gag - immunology HIV vaccines Human immunodeficiency virus IL-13Rα2/IL-4R antagonist adjuvants Integrins Lymphocytes Mice, Inbred BALB C Microarray Analysis Mucosal biomarkers Perforin Principal components analysis Rodents Single cell analysis Spleen Spleen - immunology Studies Vaccines
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Beschreibung
Zusammenfassung:	Highlights • RANTES, MIP-1β, perforin, α4, β1 and β7: set of biomarkers to measure HIV-specific mucosal immunity. • Intensity of IL-13 inhibition can modulate the level of poly-functionality and CTL immunity. • According to the anatomical location immune markers expressed can be significantly different. • According to the anatomical location Em and Cm T cell ratios can be significantly different. • Fluidigm 48.48 dynamic array is an effective tool to measure vaccine-specific mucosal immunity.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2015.10.085