Ghrelin, neuropeptide Y (NPY) and cholecystokinin (CCK) in blunt snout bream ( Megalobrama amblycephala ): cDNA cloning, tissue distribution and mRNA expression changes responding to fasting and refeeding

Highlights • Full-length cDNAs of ghrelin, NPY and CCK were cloned in blunt snout bream. • Deduced amino acid sequences of ghrelin, NPY and CCK were analyzed. • Ghrelin was mainly expressed in the intestine, and NPY and CCK in the brain. • Ghrelin and NPY had opposite role of CCK responding to fasti...

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Veröffentlicht in:	General and comparative endocrinology 2015-11, Vol.223, p.108-119
Hauptverfasser:	Ji, Wei, Ping, Hai-Chao, Wei, Kai-Jian, Zhang, Gui-Rong, Shi, Ze-Chao, Yang, Rui-Bin, Zou, Gui-Wei, Wang, Wei-Min
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Base Sequence Cholecystokinin Cholecystokinin - genetics Cholecystokinin - metabolism Cloning, Molecular Ctenopharyngodon idella Cyprinidae - genetics Cyprinidae - growth & development Cyprinidae - metabolism DNA, Complementary - genetics Endocrinology & Metabolism Fasting Fasting - physiology Feeding Behavior - physiology Fish Proteins - genetics Fish Proteins - metabolism Freshwater Ghrelin Ghrelin - genetics Ghrelin - metabolism Megalobrama amblycephala Molecular Sequence Data Neuropeptide Y Neuropeptide Y - genetics Neuropeptide Y - metabolism Real-Time Polymerase Chain Reaction Refeeding Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Sequence Homology, Amino Acid Teleostei Tissue Distribution
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Zusammenfassung:	Highlights • Full-length cDNAs of ghrelin, NPY and CCK were cloned in blunt snout bream. • Deduced amino acid sequences of ghrelin, NPY and CCK were analyzed. • Ghrelin was mainly expressed in the intestine, and NPY and CCK in the brain. • Ghrelin and NPY had opposite role of CCK responding to fasting and refeeding.
ISSN:	0016-6480 1095-6840
DOI:	10.1016/j.ygcen.2015.08.009