BODIPY–Coumarin Conjugate as an Endoplasmic Reticulum Membrane Fluidity Sensor and Its Application to ER Stress Models

An endoplasmic reticulum (ER) membrane-selective chemosensor composed of BODIPY and coumarin moieties and a long alkyl chain (n-C18) was synthesized. The emission ratio of BODIPY to coumarin depends on the solution viscosity. The probe is localized to the ER membrane and was applied to reveal the re...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Bioconjugate chemistry 2015-12, Vol.26 (12), p.2474-2480
Hauptverfasser:	Lee, Hoyeon, Yang, Zhigang, Wi, Youngjin, Kim, Tae Woo, Verwilst, Peter, Lee, Yun Hak, Han, Ga-in, Kang, Chulhun, Kim, Jong Seung
Format:	Artikel
Sprache:	eng
Schlagworte:	Aqueous solutions Biochemistry Boron Compounds - chemistry Coumarins - chemistry Endoplasmic Reticulum - chemistry Endoplasmic Reticulum - metabolism Endoplasmic Reticulum Stress Fluorescent Dyes - chemistry HeLa Cells Humans Membrane Fluidity Membranes Microscopy, Fluorescence Spectrometry, Fluorescence Viscosity
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	An endoplasmic reticulum (ER) membrane-selective chemosensor composed of BODIPY and coumarin moieties and a long alkyl chain (n-C18) was synthesized. The emission ratio of BODIPY to coumarin depends on the solution viscosity. The probe is localized to the ER membrane and was applied to reveal the reduced ER membrane fluidity under ER stress conditions.
ISSN:	1043-1802 1520-4812
DOI:	10.1021/acs.bioconjchem.5b00508