A simple method for monitoring chromatography column eluates for laccase activity during enzyme purification

A simple method for monitoring chromatography column eluates for laccase activity during enzyme purification

A simple and economical method is described that allows rapid detection of laccase activity in chromatography column fractions during enzyme purification. Aliquots of column eluants are applied to filter paper coated with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) containing a...

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Veröffentlicht in:Biotechnology letters 2000-01, Vol.22 (2), p.105-108
Hauptverfasser: DEKKER, R. F. H, KHU YEE LING, BARBOSA, A. M
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Biotechnology
Enzyme engineering
Fundamental and applied biological sciences. Psychology
Improved methods for extraction and purification of enzymes
Methods. Procedures. Technologies
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Zusammenfassung:A simple and economical method is described that allows rapid detection of laccase activity in chromatography column fractions during enzyme purification. Aliquots of column eluants are applied to filter paper coated with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) containing a numbered grid, and incubated at ambient temperature for 20 min. Indications of enzyme activity are simply observed by a colour change. This method avoids having to manually assay each fraction of a chromatographic run for enzyme activity.
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005680027798