Effects of 2-Deoxy-D-Glucose Administration on Cytokine Production in BDF sub(1) Mice

Physical exercise and diet changes have been shown to affect immune parameters, and similar effects are also induced by the administration of a nonmetabolizable glucose analog, 2-deoxy-D-glucose (2-DG). The present study was designed to characterize the effects of glucoprivation induced by 2-DG administration on concentrations of tumor necrosis factor- alpha (TNF- alpha ), interleukin-1 beta (IL-1 beta ), and IL-6 in the blood and interferon- gamma (IFN- gamma ), IL-2, and IL-4 in vitro production by partially purified T splenocytes in BDF sub(1) mice. Mice (n = 8 per group) were injected intraperitoneally one or three times with 0, 500, 750, or 1000 mg/kg of 2-DG, and blood and spleens were collected 2 h after the last injection. Partially purified T splenocytes were cultured 24 h in the presence of concanavalin A (ConA). A significant increase in the corticosterone levels with the amount of 2-DG injected was observed after one or three injections (p < 0.05). The amount of 2-DG injected was associated with an increase in TNF- alpha , IL-1 beta , and IL-6 concentrations in the blood of mice after one or three injections of 2-DG (p < 0.05). A significant decrease in in vitro proliferation of partially purified splenocytes in the presence of ConA was associated with a decrease in IFN- gamma production in the culture supernatants and an increase in IL-1 receptor expression on the cell surface (p < 0.05).