Advancing forensic RNA typing: On non-target secretions, a nasal mucosa marker, a differential co-extraction protocol and the sensitivity of DNA and RNA profiling

Advancing forensic RNA typing: On non-target secretions, a nasal mucosa marker, a differential co-extraction protocol and the sensitivity of DNA and RNA profiling

Highlights • A nasal mucosa marker is described. • An overview of mRNA marker expression in non-target tissues is given. • Microbial markers are assessed for their suitability to identify vaginal mucosa. • Relative sensitivities of DNA and RNA profiling results are examined. • Variability of mRNA ma...

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Veröffentlicht in:Forensic science international : genetics 2016-01, Vol.20, p.119-129
Hauptverfasser: van den Berge, Margreet, Bhoelai, Bryan, Harteveld, Joyce, Matai, Anuska, Sijen, Titia
Format: Artikel
Sprache:eng
Schlagworte:
Biomarkers - analysis
Body fluid identification
Body Fluids - chemistry
DNA - analysis
DNA - genetics
DNA Fingerprinting - methods
Female
Forensic Genetics - methods
Forensic science
Gene Expression Profiling - methods
Humans
Male
Menstruation - metabolism
mRNA profiling
Nasal mucosa
Nasal Mucosa - chemistry
Pathology
RNA - analysis
RNA - genetics
RNA profile interpretation
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Saliva - chemistry
Semen - chemistry
Skin - chemistry
Vagina - microbiology
Vagina - secretion
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Beschreibung
Zusammenfassung:Highlights • A nasal mucosa marker is described. • An overview of mRNA marker expression in non-target tissues is given. • Microbial markers are assessed for their suitability to identify vaginal mucosa. • Relative sensitivities of DNA and RNA profiling results are examined. • Variability of mRNA marker detection in RNA profiling replicates is discussed.
ISSN:1872-4973
1878-0326
DOI:10.1016/j.fsigen.2015.10.011