Production of high titer attenuated poliovirus strains on the serum-free PER.C6® cell culture platform for the generation of safe and affordable next generation IPV

Highlights • PER.C6® cells are susceptible to infection with attenuated poliovirus strains. • Sabin strains in PER.C6® cells show increased productivity as compared to Vero cells. • Sabin strain productivity in PER.C6® cells was maintained during infection at 10 l scale. • Rationally-attenuated stra...

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Veröffentlicht in:	Vaccine 2015-11, Vol.33 (48), p.6611-6616
Hauptverfasser:	Sanders, Barbara P, Oakes, Isabel de los Rios, van Hoek, Vladimir, Liu, Ying, Marissen, Wilfred, Minor, Philip D, Wimmer, Eckard, Schuitemaker, Hanneke, Custers, Jerome H.H.V, Macadam, Andrew, Cello, Jeronimo, Edo-Matas, Diana
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology Animals Antibodies, Viral - blood Cell Culture Techniques Cell Line Cercopithecus aethiops Culture Media, Serum-Free - chemistry Enzyme-Linked Immunosorbent Assay Manufacturing Mutation Next generation IPV PER.C6® cells Poliomyelitis Poliomyelitis - prevention & control Poliovirus Poliovirus - genetics Poliovirus - growth & development Poliovirus Vaccine, Inactivated - immunology Poliovirus Vaccine, Oral Rabbits Rationally-attenuated strains Sabin Vaccines Vaccines, Attenuated Vero Cells Viral Load Virus Cultivation - methods
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Beschreibung
Zusammenfassung:	Highlights • PER.C6® cells are susceptible to infection with attenuated poliovirus strains. • Sabin strains in PER.C6® cells show increased productivity as compared to Vero cells. • Sabin strain productivity in PER.C6® cells was maintained during infection at 10 l scale. • Rationally-attenuated strains demonstrated high yields on the PER.C6® platform.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2015.10.091