Preparation of algal-oligosaccharide mixtures by bacterial agarases and their antioxidative properties

Algal-oligosaccharide-lysates (AOL), derived from six agars and tour algal polysaccharide extracts (APE), were treated with 100-500 activity units (AU) of MA1O3-agarases or MAEF108-agarases, and their antioxidative properties evaluated. Soluble total polyphenols (TP) were between 462.2 +-- 1.6 gallic acid equivalents (GAE. Microg/mL) and 70.6 +-- 17.4 GAE. The DPPH radical scavenging capacity of all AOL went from 68.3 +-- 0.7+ACU- to 0.5 +-- 0.1+ACU-. The ferrous ion chelating capacity of all AOL went from 93.1 +-- 0.2+ACU- to 21.7 +--0.9+ACU-. Evaluation of the H2O2 scavenging capacity of all AOL was between 35.9 +-- 5.4+ACU- and 0.1 +-- 0.2+ACU-. The reducing power of all AOL went from 51.3 +-- 2.6 to 3.2 +-- 6.8 expressed as Microg/mL ascorbic acid. In DPPH radical scavenging capacity, ferrous ion chelating capacity and reducing power etc., the AOL derived from the APE of Porphyra dentate (digested by 500 AU of MAEF1O8-agarases) were highest, in all test sets. However, the AOL derived from the APE of Monostroma nitidum (digested by 500 AU of MAEF1O8-agarases) had the highest H2O2 scavenging capacity in all test sets. The order of antioxidative activity performance of all AOL treated in this experiment, by these four antioxidative methods, is as follows: ferrous ion chelating capacity +AD4- DPPH radical scavenging capacity +AD4- H2O2 scavenging capacity +AD4- reducing power+ADs- this may be related to their polyphenols, small molecular weight polysaccharides or simple sugar constituents. In this study, it is demonstrated that various agarases derived from algal oligosaccharide mixtures possess good potential for use as a health food, due to their antioxidative capacity.