Methyl 3-Hydroxymyristate, a Diffusible Signal Mediating phc Quorum Sensing in Ralstonia solanacearum
Ralstonia solanacearum, a plant pathogenic bacterium causing “bacterial wilt” on crops, uses a quorum sensing (QS) system consisting of phc regulatory elements to control its virulence. Methyl 3‐hydroxypalmitate (3‐OH PAME) was previously identified as the QS signal in strain AW1. However, 3‐OH PAME...
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| Veröffentlicht in: | Chembiochem : a European journal of chemical biology 2015-11, Vol.16 (16), p.2309-2318 |
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| creator | Kai, Kenji Ohnishi, Hideyuki Shimatani, Mika Ishikawa, Shiho Mori, Yuka Kiba, Akinori Ohnishi, Kouhei Tabuchi, Mitsuaki Hikichi, Yasufumi |
| description | Ralstonia solanacearum, a plant pathogenic bacterium causing “bacterial wilt” on crops, uses a quorum sensing (QS) system consisting of phc regulatory elements to control its virulence. Methyl 3‐hydroxypalmitate (3‐OH PAME) was previously identified as the QS signal in strain AW1. However, 3‐OH PAME has not been reportedly detected from any other strains, and this suggests that they produce another unknown QS signal. Here we identify (R)‐methyl 3‐hydroxymyristate [(R)‐3‐OH MAME] as a new QS signal that regulates the production of virulence factors and secondary metabolites. (R)‐3‐OH MAME was synthesized by the methyltransferase PhcB and sensed by the histidine kinase PhcS. The phylogenetic trees of these proteins from R. solanacearum strains were divided into two groups, according to their QS signal types—(R)‐3‐OH MAME or (R)‐3‐OH PAME. These results demonstrate that (R)‐3‐OH MAME is another crucial QS signal and highlight the unique evolution of QS systems in R. solanacearum.
Chemical signaling in Ralstonia: We report that (R)‐methyl 3‐hydroxymyristate [(R)‐3‐OH MAME] is a quorum sensing (QS) signal that regulates the production of virulence factors in Ralstonia solanacearum strain OE1‐1. (R)‐3‐OH MAME was also produced by many other strains. Thus, the compound is another Ralstonia QS signal that has been overlooked for nearly two decades. |
| doi_str_mv | 10.1002/cbic.201500456 |
| format | Article |
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Chemical signaling in Ralstonia: We report that (R)‐methyl 3‐hydroxymyristate [(R)‐3‐OH MAME] is a quorum sensing (QS) signal that regulates the production of virulence factors in Ralstonia solanacearum strain OE1‐1. (R)‐3‐OH MAME was also produced by many other strains. Thus, the compound is another Ralstonia QS signal that has been overlooked for nearly two decades.</description><identifier>ISSN: 1439-4227</identifier><identifier>EISSN: 1439-7633</identifier><identifier>DOI: 10.1002/cbic.201500456</identifier><identifier>PMID: 26360813</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Bacterial Proteins - classification ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacteriology ; Biological Products - chemistry ; Biological Products - metabolism ; diffusible signals ; Gas Chromatography-Mass Spectrometry ; Kinases ; Myristates - chemistry ; Myristates - isolation & purification ; Myristates - metabolism ; natural products ; Palmitic Acids - chemistry ; Palmitic Acids - metabolism ; Phylogeny ; quorum sensing ; Quorum Sensing - genetics ; Quorum Sensing - physiology ; Ralstonia solanacearum ; Ralstonia solanacearum - genetics ; Ralstonia solanacearum - metabolism ; Regulatory Elements, Transcriptional - genetics ; Stereoisomerism ; structure elucidation ; Structure-Activity Relationship ; Virulence Factors - genetics ; Virulence Factors - metabolism</subject><ispartof>Chembiochem : a European journal of chemical biology, 2015-11, Vol.16 (16), p.2309-2318</ispartof><rights>2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5476-e7baa974be43ccabe164acc211e4bf927bb6fa26037e46781f0b96aa1f82b28d3</citedby><cites>FETCH-LOGICAL-c5476-e7baa974be43ccabe164acc211e4bf927bb6fa26037e46781f0b96aa1f82b28d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbic.201500456$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbic.201500456$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26360813$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kai, Kenji</creatorcontrib><creatorcontrib>Ohnishi, Hideyuki</creatorcontrib><creatorcontrib>Shimatani, Mika</creatorcontrib><creatorcontrib>Ishikawa, Shiho</creatorcontrib><creatorcontrib>Mori, Yuka</creatorcontrib><creatorcontrib>Kiba, Akinori</creatorcontrib><creatorcontrib>Ohnishi, Kouhei</creatorcontrib><creatorcontrib>Tabuchi, Mitsuaki</creatorcontrib><creatorcontrib>Hikichi, Yasufumi</creatorcontrib><title>Methyl 3-Hydroxymyristate, a Diffusible Signal Mediating phc Quorum Sensing in Ralstonia solanacearum</title><title>Chembiochem : a European journal of chemical biology</title><addtitle>ChemBioChem</addtitle><description>Ralstonia solanacearum, a plant pathogenic bacterium causing “bacterial wilt” on crops, uses a quorum sensing (QS) system consisting of phc regulatory elements to control its virulence. Methyl 3‐hydroxypalmitate (3‐OH PAME) was previously identified as the QS signal in strain AW1. However, 3‐OH PAME has not been reportedly detected from any other strains, and this suggests that they produce another unknown QS signal. Here we identify (R)‐methyl 3‐hydroxymyristate [(R)‐3‐OH MAME] as a new QS signal that regulates the production of virulence factors and secondary metabolites. (R)‐3‐OH MAME was synthesized by the methyltransferase PhcB and sensed by the histidine kinase PhcS. The phylogenetic trees of these proteins from R. solanacearum strains were divided into two groups, according to their QS signal types—(R)‐3‐OH MAME or (R)‐3‐OH PAME. These results demonstrate that (R)‐3‐OH MAME is another crucial QS signal and highlight the unique evolution of QS systems in R. solanacearum.
Chemical signaling in Ralstonia: We report that (R)‐methyl 3‐hydroxymyristate [(R)‐3‐OH MAME] is a quorum sensing (QS) signal that regulates the production of virulence factors in Ralstonia solanacearum strain OE1‐1. (R)‐3‐OH MAME was also produced by many other strains. Thus, the compound is another Ralstonia QS signal that has been overlooked for nearly two decades.</description><subject>Bacterial Proteins - classification</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Biological Products - chemistry</subject><subject>Biological Products - metabolism</subject><subject>diffusible signals</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Kinases</subject><subject>Myristates - chemistry</subject><subject>Myristates - isolation & purification</subject><subject>Myristates - metabolism</subject><subject>natural products</subject><subject>Palmitic Acids - chemistry</subject><subject>Palmitic Acids - metabolism</subject><subject>Phylogeny</subject><subject>quorum sensing</subject><subject>Quorum Sensing - genetics</subject><subject>Quorum Sensing - physiology</subject><subject>Ralstonia solanacearum</subject><subject>Ralstonia solanacearum - genetics</subject><subject>Ralstonia solanacearum - metabolism</subject><subject>Regulatory Elements, Transcriptional - genetics</subject><subject>Stereoisomerism</subject><subject>structure elucidation</subject><subject>Structure-Activity Relationship</subject><subject>Virulence Factors - genetics</subject><subject>Virulence Factors - metabolism</subject><issn>1439-4227</issn><issn>1439-7633</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvEzEURkcIRB-wZYkssWHRCX6NPbOkAZqiBlQCYmldO3dal3kEe0Z0_j0OCRFi05Ut-3yfrn2y7AWjM0Ypf-OsdzNOWUGpLNSj7JhJUeVaCfF4v5ec66PsJMY7SmmlBHuaHXElFC2ZOM5wicPt1BCRL6Z16O-ndgo-DjDgGQHyztf1GL1tkKz8TQcNWeLaw-C7G7K5deR67MPYkhV2cXvkO_IFmjj0nQcS-wY6cAiJeJY9qdMFPt-vp9m3D--_zhf51eeLy_nbq9wVUqsctQWotLQohXNgkSkJznHGUNq64tpaVQNXVGiUSpesprZSAKwuueXlWpxmr3e9m9D_HDEOpvXRYZMmwX6MhmmpykoV6ekPo7ykhaRlmdBX_6F3_RjSb_yhpCqEVDJRsx3lQh9jwNpsgm8hTIZRs3Vltq7MwVUKvNzXjrbF9QH_KycB1Q745RucHqgz8_PL-b_l-S6bZOL9IQvhh1Fa6MJ8_3Rhlgv-caGul2YlfgMj369h</recordid><startdate>20151102</startdate><enddate>20151102</enddate><creator>Kai, Kenji</creator><creator>Ohnishi, Hideyuki</creator><creator>Shimatani, Mika</creator><creator>Ishikawa, Shiho</creator><creator>Mori, Yuka</creator><creator>Kiba, Akinori</creator><creator>Ohnishi, Kouhei</creator><creator>Tabuchi, Mitsuaki</creator><creator>Hikichi, Yasufumi</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20151102</creationdate><title>Methyl 3-Hydroxymyristate, a Diffusible Signal Mediating phc Quorum Sensing in Ralstonia solanacearum</title><author>Kai, Kenji ; 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Methyl 3‐hydroxypalmitate (3‐OH PAME) was previously identified as the QS signal in strain AW1. However, 3‐OH PAME has not been reportedly detected from any other strains, and this suggests that they produce another unknown QS signal. Here we identify (R)‐methyl 3‐hydroxymyristate [(R)‐3‐OH MAME] as a new QS signal that regulates the production of virulence factors and secondary metabolites. (R)‐3‐OH MAME was synthesized by the methyltransferase PhcB and sensed by the histidine kinase PhcS. The phylogenetic trees of these proteins from R. solanacearum strains were divided into two groups, according to their QS signal types—(R)‐3‐OH MAME or (R)‐3‐OH PAME. These results demonstrate that (R)‐3‐OH MAME is another crucial QS signal and highlight the unique evolution of QS systems in R. solanacearum.
Chemical signaling in Ralstonia: We report that (R)‐methyl 3‐hydroxymyristate [(R)‐3‐OH MAME] is a quorum sensing (QS) signal that regulates the production of virulence factors in Ralstonia solanacearum strain OE1‐1. (R)‐3‐OH MAME was also produced by many other strains. Thus, the compound is another Ralstonia QS signal that has been overlooked for nearly two decades.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>26360813</pmid><doi>10.1002/cbic.201500456</doi><tpages>10</tpages></addata></record> |
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| subjects | Bacterial Proteins - classification Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Biological Products - chemistry Biological Products - metabolism diffusible signals Gas Chromatography-Mass Spectrometry Kinases Myristates - chemistry Myristates - isolation & purification Myristates - metabolism natural products Palmitic Acids - chemistry Palmitic Acids - metabolism Phylogeny quorum sensing Quorum Sensing - genetics Quorum Sensing - physiology Ralstonia solanacearum Ralstonia solanacearum - genetics Ralstonia solanacearum - metabolism Regulatory Elements, Transcriptional - genetics Stereoisomerism structure elucidation Structure-Activity Relationship Virulence Factors - genetics Virulence Factors - metabolism |
| title | Methyl 3-Hydroxymyristate, a Diffusible Signal Mediating phc Quorum Sensing in Ralstonia solanacearum |
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