Fine mapping of S37, a locus responsible for pollen and embryo sac sterility in hybrids between Oryza sativa L. and O. glaberrima Steud
Key message Hybrid sterility locus S37 between Oryza glaberrima and Oryza sativa results in both pollen and embryo sac sterility. Interspecific crossing between African cultivated rice Oryza glaberrima and Oryza sativa cultivars is hindered by hybrid sterility. To dissect the mechanism of interspeci...
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Veröffentlicht in: | Plant cell reports 2015-11, Vol.34 (11), p.1885-1897 |
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Hybrid sterility locus
S37
between
Oryza glaberrima
and
Oryza sativa
results in both pollen and embryo sac sterility.
Interspecific crossing between African cultivated rice
Oryza glaberrima
and
Oryza sativa
cultivars is hindered by hybrid sterility. To dissect the mechanism of interspecific hybrid sterility, we developed a near-isogenic line (NIL)-
S37
using Dianjingyou1 (DJY1) as the recipient parent and an African cultivated rice variety as the donor parent. Empty pollen and embryo sac sterility were observed in F
1
hybrids between DJY1 and NIL-
S37
. Cytological analyses showed that pollen abortion in the F
1
hybrids occurred at the late binucleate stage due to a failure of starch accumulation in pollen grains. In addition, partial abortion of the embryo sac in the F
1
hybrid was observed during function megaspore developing into mature embryo sac. Molecular analysis revealed that the semi-sterility was largely caused by the abortion of male and female gametophytes carrying the
S37
allele from DJY1. A population of 25,600 plants derived from the hybrid DJY1/NIL-
S37
was developed to fine map
S37
. Based on the physical location of molecular markers,
S37
locus was finally delimited to a region of 205 kb on the short arm of chromosome 1 in terms of reference sequences of cv. Nipponbare. Interestingly, an about 97-kb DNA segment was deleted in the NIL-
S37
based on BAC clone information of
O.
glaberrima
. Fifty-four open reading frames (ORF) were predicted in this 205-kb region of DJY1, whereas only 31 ORFs were in that of NIL-
S37
. These results are valuable for cloning of
S37
gene and further breaking reproductive isolation between
Oryza glaberrima
and
Oryza sativa
cultivars, as well as marker-assisted transferring of the corresponding neutral allele in rice breeding programs. |
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ISSN: | 0721-7714 1432-203X |
DOI: | 10.1007/s00299-015-1835-4 |