Gene expression profiles of FABP genes in protochordates, Ciona intestinalis and Branchiostoma belcheri
Fatty-acid-binding proteins (FABPs) are small intracellular proteins associated with the transportation of fatty acids. Members of the FABPs share similar amino acid sequences and tertiary structures and form, together with a member of the cellular retinol-binding proteins (CRBPs), the intracellular...
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description | Fatty-acid-binding proteins (FABPs) are small intracellular proteins associated with the transportation of fatty acids. Members of the FABPs share similar amino acid sequences and tertiary structures and form, together with a member of the cellular retinol-binding proteins (CRBPs), the intracellular-lipid-binding protein (iLBP) family. In vertebrates, several types of FABP have been isolated and classified into three subfamilies: 2–4. In invertebrates, several FABP-related proteins have been reported in protostomes and amphioxus; however, little is known about the relationship between their phylogenetic positions and expression patterns. We have performed a genome-wide survey of FABP-related genes in protochordates: amphioxus Branchiostoma belcheri and the ascidian Ciona intestinalis. Comprehensive BLAST searches in NCBI and the Ciona Ghost Database by using amino acid sequences of all FABPs have revealed that the ascidian C. intestinalis and amphioxus B. belcheri contain six and seven FABP-related genes in their haploid genomes, respectively. Expression pattern analyses by whole-mount in situ hybridization in Ciona transparent juveniles and serial-section in situ hybridizations in adult amphioxus have revealed that all genes are mainly expressed in the postpharyngeal digestive tract. In particular, the expression of FABP-related genes of subfamily-2 (liver/ileum type) and subfamily-3 (intestinal type) in the ascidian pyloric gland and amphioxus hepatic cecum provides insight into the evolution of hepatic-related structures of chordates and FABP-related genes. |
doi_str_mv | 10.1007/s00441-015-2198-4 |
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Members of the FABPs share similar amino acid sequences and tertiary structures and form, together with a member of the cellular retinol-binding proteins (CRBPs), the intracellular-lipid-binding protein (iLBP) family. In vertebrates, several types of FABP have been isolated and classified into three subfamilies: 2–4. In invertebrates, several FABP-related proteins have been reported in protostomes and amphioxus; however, little is known about the relationship between their phylogenetic positions and expression patterns. We have performed a genome-wide survey of FABP-related genes in protochordates: amphioxus Branchiostoma belcheri and the ascidian Ciona intestinalis. Comprehensive BLAST searches in NCBI and the Ciona Ghost Database by using amino acid sequences of all FABPs have revealed that the ascidian C. intestinalis and amphioxus B. belcheri contain six and seven FABP-related genes in their haploid genomes, respectively. Expression pattern analyses by whole-mount in situ hybridization in Ciona transparent juveniles and serial-section in situ hybridizations in adult amphioxus have revealed that all genes are mainly expressed in the postpharyngeal digestive tract. In particular, the expression of FABP-related genes of subfamily-2 (liver/ileum type) and subfamily-3 (intestinal type) in the ascidian pyloric gland and amphioxus hepatic cecum provides insight into the evolution of hepatic-related structures of chordates and FABP-related genes.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/s00441-015-2198-4</identifier><identifier>PMID: 25957647</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>adults ; Amino Acid Sequence - genetics ; amino acid sequences ; amino acids ; Animals ; Biological Evolution ; Biomedical and Life Sciences ; Biomedicine ; Branchiostoma belcheri ; Branchiostoma lanceolatum ; cecum ; Chordata ; Ciona intestinalis ; Ciona intestinalis - genetics ; digestive tract ; family ; fatty acid-binding proteins ; Fatty Acid-Binding Proteins - genetics ; Fatty acids ; Gene expression ; genes ; Genome ; Genomes ; haploidy ; Human Genetics ; hybridization ; ileum ; in situ hybridization ; invertebrates ; juveniles ; Lancelets - genetics ; liver ; Liver - metabolism ; Molecular Medicine ; Phylogeny ; Proteins ; Proteomics ; Regular Article ; Retinol-Binding Proteins, Cellular - genetics ; Retinol-Binding Proteins, Cellular - metabolism ; Sequence Homology, Amino Acid ; surveys ; transportation ; vertebrates</subject><ispartof>Cell and tissue research, 2015-11, Vol.362 (2), p.331-345</ispartof><rights>Springer-Verlag Berlin Heidelberg 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-feea5a8cf440f24efdd29e832ee071647c90b936a3dbae7cb068329f6a13b29f3</citedby><cites>FETCH-LOGICAL-c462t-feea5a8cf440f24efdd29e832ee071647c90b936a3dbae7cb068329f6a13b29f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00441-015-2198-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00441-015-2198-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27911,27912,41475,42544,51306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25957647$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Orito, Wataru</creatorcontrib><creatorcontrib>Ohhira, Fuyuko</creatorcontrib><creatorcontrib>Ogasawara, Michio</creatorcontrib><title>Gene expression profiles of FABP genes in protochordates, Ciona intestinalis and Branchiostoma belcheri</title><title>Cell and tissue research</title><addtitle>Cell Tissue Res</addtitle><addtitle>Cell Tissue Res</addtitle><description>Fatty-acid-binding proteins (FABPs) are small intracellular proteins associated with the transportation of fatty acids. Members of the FABPs share similar amino acid sequences and tertiary structures and form, together with a member of the cellular retinol-binding proteins (CRBPs), the intracellular-lipid-binding protein (iLBP) family. In vertebrates, several types of FABP have been isolated and classified into three subfamilies: 2–4. In invertebrates, several FABP-related proteins have been reported in protostomes and amphioxus; however, little is known about the relationship between their phylogenetic positions and expression patterns. We have performed a genome-wide survey of FABP-related genes in protochordates: amphioxus Branchiostoma belcheri and the ascidian Ciona intestinalis. Comprehensive BLAST searches in NCBI and the Ciona Ghost Database by using amino acid sequences of all FABPs have revealed that the ascidian C. intestinalis and amphioxus B. belcheri contain six and seven FABP-related genes in their haploid genomes, respectively. Expression pattern analyses by whole-mount in situ hybridization in Ciona transparent juveniles and serial-section in situ hybridizations in adult amphioxus have revealed that all genes are mainly expressed in the postpharyngeal digestive tract. In particular, the expression of FABP-related genes of subfamily-2 (liver/ileum type) and subfamily-3 (intestinal type) in the ascidian pyloric gland and amphioxus hepatic cecum provides insight into the evolution of hepatic-related structures of chordates and FABP-related genes.</description><subject>adults</subject><subject>Amino Acid Sequence - genetics</subject><subject>amino acid sequences</subject><subject>amino acids</subject><subject>Animals</subject><subject>Biological Evolution</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Branchiostoma belcheri</subject><subject>Branchiostoma lanceolatum</subject><subject>cecum</subject><subject>Chordata</subject><subject>Ciona intestinalis</subject><subject>Ciona intestinalis - genetics</subject><subject>digestive tract</subject><subject>family</subject><subject>fatty acid-binding proteins</subject><subject>Fatty Acid-Binding Proteins - genetics</subject><subject>Fatty acids</subject><subject>Gene expression</subject><subject>genes</subject><subject>Genome</subject><subject>Genomes</subject><subject>haploidy</subject><subject>Human Genetics</subject><subject>hybridization</subject><subject>ileum</subject><subject>in situ hybridization</subject><subject>invertebrates</subject><subject>juveniles</subject><subject>Lancelets - genetics</subject><subject>liver</subject><subject>Liver - metabolism</subject><subject>Molecular Medicine</subject><subject>Phylogeny</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Regular Article</subject><subject>Retinol-Binding Proteins, Cellular - genetics</subject><subject>Retinol-Binding Proteins, Cellular - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><subject>surveys</subject><subject>transportation</subject><subject>vertebrates</subject><issn>0302-766X</issn><issn>1432-0878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU-LFDEQxYMo7rj6AbxowIsHWyt_Ot193B3cVVhQ0AVvIZ2uzGTp6YxJD-i3t3Z6FfGg5lKE96uqRz3Gngp4LQCaNwVAa1GBqCspurbS99hKaCUraJv2PluBAlk1xnw5YY9KuQEQ2pjuITuRdVc3RjcrtrnECTl-22csJaaJ73MKccTCU-AXZ-cf-YaAwuNRmZPfpjy4GcsrvibckUCfOU5ujIW7aeDn2U1-G1OZ087xHke_xRwfswfBjQWf3NVTdn3x9vP6XXX14fL9-uyq8trIuQqIrnatD1pDkBrDMMgOWyURoRHk2HfQd8o4NfQOG9-DIbELxgnVU1Wn7OUyl9x-PZAzu4vF4zi6CdOhWAn06loL809UNNq0Ld2p_Q9USQVEAqEv_kBv0iHTeY4Ure3UkRIL5XMqJWOw-xx3Ln-3AuxttnbJ1lK29jZbq6nn2d3kQ7_D4VfHzzAJkAtQSJo2mH9b_Zepz5em4JJ1mxyLvf4kQRi6k27azqgf2YK3iw</recordid><startdate>20151101</startdate><enddate>20151101</enddate><creator>Orito, Wataru</creator><creator>Ohhira, Fuyuko</creator><creator>Ogasawara, Michio</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20151101</creationdate><title>Gene expression profiles of FABP genes in protochordates, Ciona intestinalis and Branchiostoma belcheri</title><author>Orito, Wataru ; Ohhira, Fuyuko ; Ogasawara, Michio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-feea5a8cf440f24efdd29e832ee071647c90b936a3dbae7cb068329f6a13b29f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>adults</topic><topic>Amino Acid Sequence - genetics</topic><topic>amino acid sequences</topic><topic>amino acids</topic><topic>Animals</topic><topic>Biological Evolution</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Branchiostoma belcheri</topic><topic>Branchiostoma lanceolatum</topic><topic>cecum</topic><topic>Chordata</topic><topic>Ciona intestinalis</topic><topic>Ciona intestinalis - genetics</topic><topic>digestive tract</topic><topic>family</topic><topic>fatty acid-binding proteins</topic><topic>Fatty Acid-Binding Proteins - genetics</topic><topic>Fatty acids</topic><topic>Gene expression</topic><topic>genes</topic><topic>Genome</topic><topic>Genomes</topic><topic>haploidy</topic><topic>Human Genetics</topic><topic>hybridization</topic><topic>ileum</topic><topic>in situ hybridization</topic><topic>invertebrates</topic><topic>juveniles</topic><topic>Lancelets - genetics</topic><topic>liver</topic><topic>Liver - metabolism</topic><topic>Molecular Medicine</topic><topic>Phylogeny</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Regular Article</topic><topic>Retinol-Binding Proteins, Cellular - 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Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Cell and tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Orito, Wataru</au><au>Ohhira, Fuyuko</au><au>Ogasawara, Michio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene expression profiles of FABP genes in protochordates, Ciona intestinalis and Branchiostoma belcheri</atitle><jtitle>Cell and tissue research</jtitle><stitle>Cell Tissue Res</stitle><addtitle>Cell Tissue Res</addtitle><date>2015-11-01</date><risdate>2015</risdate><volume>362</volume><issue>2</issue><spage>331</spage><epage>345</epage><pages>331-345</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><abstract>Fatty-acid-binding proteins (FABPs) are small intracellular proteins associated with the transportation of fatty acids. Members of the FABPs share similar amino acid sequences and tertiary structures and form, together with a member of the cellular retinol-binding proteins (CRBPs), the intracellular-lipid-binding protein (iLBP) family. In vertebrates, several types of FABP have been isolated and classified into three subfamilies: 2–4. In invertebrates, several FABP-related proteins have been reported in protostomes and amphioxus; however, little is known about the relationship between their phylogenetic positions and expression patterns. We have performed a genome-wide survey of FABP-related genes in protochordates: amphioxus Branchiostoma belcheri and the ascidian Ciona intestinalis. Comprehensive BLAST searches in NCBI and the Ciona Ghost Database by using amino acid sequences of all FABPs have revealed that the ascidian C. intestinalis and amphioxus B. belcheri contain six and seven FABP-related genes in their haploid genomes, respectively. Expression pattern analyses by whole-mount in situ hybridization in Ciona transparent juveniles and serial-section in situ hybridizations in adult amphioxus have revealed that all genes are mainly expressed in the postpharyngeal digestive tract. In particular, the expression of FABP-related genes of subfamily-2 (liver/ileum type) and subfamily-3 (intestinal type) in the ascidian pyloric gland and amphioxus hepatic cecum provides insight into the evolution of hepatic-related structures of chordates and FABP-related genes.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>25957647</pmid><doi>10.1007/s00441-015-2198-4</doi><tpages>15</tpages></addata></record> |
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subjects | adults Amino Acid Sequence - genetics amino acid sequences amino acids Animals Biological Evolution Biomedical and Life Sciences Biomedicine Branchiostoma belcheri Branchiostoma lanceolatum cecum Chordata Ciona intestinalis Ciona intestinalis - genetics digestive tract family fatty acid-binding proteins Fatty Acid-Binding Proteins - genetics Fatty acids Gene expression genes Genome Genomes haploidy Human Genetics hybridization ileum in situ hybridization invertebrates juveniles Lancelets - genetics liver Liver - metabolism Molecular Medicine Phylogeny Proteins Proteomics Regular Article Retinol-Binding Proteins, Cellular - genetics Retinol-Binding Proteins, Cellular - metabolism Sequence Homology, Amino Acid surveys transportation vertebrates |
title | Gene expression profiles of FABP genes in protochordates, Ciona intestinalis and Branchiostoma belcheri |
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