A reliable phenotypic assay for detection of ESBLs and AmpCs in MBL-producing gram-negative bacteria with the use of aminophenylboronic acid, dipicolinic acid and cloxacillin

ESBLs and AmpCs may escape detection when they coexist with metallo-β-lactamases such as New Delhi Metallo-β-lactamases-1. In this study a combination disk assay was established using cefotaxime, cefotaxime/clavulanic acid, cefotaxime/clavulanic acid/cloxacillin, cefoxitin and cefoxitin/phenylboroni...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of microbiological methods 2015-08, Vol.115, p.100-103
Hauptverfasser:	Datta, Saswati, Chatterjee, Somdatta, Mitra, Shravani, Basu, Sulagna
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Bacterial Agents - pharmacology Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacterial Typing Techniques - methods beta-Lactamases - genetics beta-Lactamases - metabolism Boronic Acids - pharmacology Cloxacillin Cloxacillin - pharmacology Combination disk test Dipicolinic acid Gram-negative bacteria Gram-Negative Bacteria - drug effects Gram-Negative Bacteria - enzymology Gram-Negative Bacteria - genetics Gram-Negative Bacteria - isolation & purification Gram-Negative Bacterial Infections - microbiology Humans NDM-1 Phenotype Phenylboronic acid Picolinic Acids - pharmacology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	ESBLs and AmpCs may escape detection when they coexist with metallo-β-lactamases such as New Delhi Metallo-β-lactamases-1. In this study a combination disk assay was established using cefotaxime, cefotaxime/clavulanic acid, cefotaxime/clavulanic acid/cloxacillin, cefoxitin and cefoxitin/phenylboronic acid/cloxacillin on Mueller Hinton agar supplemented with dipicolinic acid for determination of β-lactamases in the presence of NDM-1. [Display omitted] •MBLs interfere with the phenotypic detection of ESBLs and AmpCs when present together.•Detection of multiple β-lactamases in the presence of NDM-1 failed with commercial diagnostic disks.•A novel combination disk assay was introduced using dipicolinic acid, cloxacillin and phenylboronic acid.•Specificity and sensitivity of the assay improved over the commercial disk-tests.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2015.05.028