The use of ferromagnetic Dacron as solid-phase in chemiluminescent assays

Ferromagnetic Dacron hydrazide particles (63-74 mu m) were activated either by converting hydrazide groups to azide or binding glutaraldehyde to the hydrazide groups. Human serum albumin (HSA) was immobilised to the activated matrices and antibodies against HSA were detected by using the chemilumine...

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Veröffentlicht in:	Biotechnology techniques 1999-12, Vol.13 (12), p.919-922
Hauptverfasser:	PINHEIRO, S. M. B, CARVALHO, L. B, CHAVES, M. E. C
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology human serum albumin Methods. Procedures. Technologies Others Various methods and equipments
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Zusammenfassung:	Ferromagnetic Dacron hydrazide particles (63-74 mu m) were activated either by converting hydrazide groups to azide or binding glutaraldehyde to the hydrazide groups. Human serum albumin (HSA) was immobilised to the activated matrices and antibodies against HSA were detected by using the chemiluminescent assay. The use of these particles (either Dacron-azide or Dacron-glutaraldehyde) gave similar results compared with the paramagnetic particles commercially available from Ciba Corning.
ISSN:	0951-208X 1573-6784
DOI:	10.1023/A:1008963202258