Loop-mediated isothermal amplification for the rapid detection of Salmonella
Loop-mediated isothermal amplification (LAMP) assay detected Salmonella within 60 min. The 220 strains of 39 serotypes of Salmonella subsp. enterica and 7 strains of Salmonella enterica subsp. arizonae were amplified, but not 62 strains of 23 bacterial species other than Salmonella. The sensitivity...
Gespeichert in:
| Veröffentlicht in: | FEMS microbiology letters 2005-12, Vol.253 (1), p.155-161 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 161 |
|---|---|
| container_issue | 1 |
| container_start_page | 155 |
| container_title | FEMS microbiology letters |
| container_volume | 253 |
| creator | Hara-Kudo, Yukiko Yoshino, Manabu Kojima, Tadashi Ikedo, Masanari |
| description | Loop-mediated isothermal amplification (LAMP) assay detected
Salmonella within 60
min. The 220 strains of 39 serotypes of
Salmonella subsp.
enterica and 7 strains of
Salmonella enterica subsp.
arizonae were amplified, but not 62 strains of 23 bacterial species other than
Salmonella. The sensitivity of the LAMP assay was found to be >2.2
cfu/test tube using nine serotypes. The specificity was similar to that of a PCR assay, but the sensitivity of LAMP was greater. Both fluorescence and turbidity were able to detect the products in the LAMP assay.
S. enteritidis in a liquid egg sample artificially inoculated with the organism was detected by the LAMP assay at 2.8
cfu/test tube, although negative by PCR assay. These results indicate that the LAMP assay is a rapid, specific and sensitive detection method for
Salmonella. |
| doi_str_mv | 10.1016/j.femsle.2005.09.032 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_17449232</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1016/j.femsle.2005.09.032</oup_id><els_id>S0378109705006683</els_id><sourcerecordid>2306554582</sourcerecordid><originalsourceid>FETCH-LOGICAL-c6745-5bc557fb34472a8c18e8c331be08bf0bbfc8d6fa4ff05d2a077fd4c92f9454df3</originalsourceid><addsrcrecordid>eNqNkk-L1TAUxYMoznP0G4gWxNm1c5PmXzeCDM4oVFyMsw5pmmgebVOTVplvb97rgwEXMqtA8jvnHs4NQq8xVBgwv9xXzo5psBUBYBU0FdTkCdphJmjJGy6foh3UQpYYGnGGXqS0BwBKgD9HZ5gTSiSHHWrbEOZytL3Xi-0Ln8Ly08ZRD4Ue58E7b_Tiw1S4EIv8UkQ9-77o7WLN8T644lYPY5jsMOiX6JnTQ7KvTuc5urv-9P3qc9l-u_ly9bEtDReUlawzjAnX1ZQKoqXB0kpT17izIDsHXeeM7LnT1DlgPdEghOupaYhrKKO9q8_RxeY7x_BrtWlRo0_mkGCyYU0KC0obUpMMvvsH3Ic1TjmbIjVwxiiTB4pulIkhpWidmqMfdbxXGNSha7VXW9fq0LWCRsHR_M3JfO1ygQ-iU7kZeH8CdDJ6cFFPxqcHThABNZeZkxv3xw_2_lHD1fXXFjOWpZebNKzzY1O_3RROB6V_xBzo7pYArgE3jLBj7A8bYfMKf3sbVTLeTib_kZi3rvrg_z_iLwmjyMk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2306554582</pqid></control><display><type>article</type><title>Loop-mediated isothermal amplification for the rapid detection of Salmonella</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Alma/SFX Local Collection</source><creator>Hara-Kudo, Yukiko ; Yoshino, Manabu ; Kojima, Tadashi ; Ikedo, Masanari</creator><creatorcontrib>Hara-Kudo, Yukiko ; Yoshino, Manabu ; Kojima, Tadashi ; Ikedo, Masanari</creatorcontrib><description>Loop-mediated isothermal amplification (LAMP) assay detected
Salmonella within 60
min. The 220 strains of 39 serotypes of
Salmonella subsp.
enterica and 7 strains of
Salmonella enterica subsp.
arizonae were amplified, but not 62 strains of 23 bacterial species other than
Salmonella. The sensitivity of the LAMP assay was found to be >2.2
cfu/test tube using nine serotypes. The specificity was similar to that of a PCR assay, but the sensitivity of LAMP was greater. Both fluorescence and turbidity were able to detect the products in the LAMP assay.
S. enteritidis in a liquid egg sample artificially inoculated with the organism was detected by the LAMP assay at 2.8
cfu/test tube, although negative by PCR assay. These results indicate that the LAMP assay is a rapid, specific and sensitive detection method for
Salmonella.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/j.femsle.2005.09.032</identifier><identifier>PMID: 16242860</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Elsevier B.V</publisher><subject>Assaying ; Bacteriological methods and techniques used in bacteriology ; Bacteriological Techniques ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Detection ; DNA, Bacterial - genetics ; DNA, Bacterial - isolation & purification ; Fluorescence ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; Genetics ; LAMP ; Microbiology ; Molecular Sequence Data ; Nucleic Acid Amplification Techniques - methods ; Nucleic Acid Amplification Techniques - statistics & numerical data ; Polymerase Chain Reaction ; Rapid ; Salmonella ; Salmonella - classification ; Salmonella - genetics ; Salmonella - isolation & purification ; Salmonella enterica ; Salmonella enterica - classification ; Salmonella enterica - genetics ; Salmonella enterica - isolation & purification ; Sensitive ; Sensitivity ; Sensitivity and Specificity ; Serotypes ; Serotyping ; Strains (organisms) ; Turbidity</subject><ispartof>FEMS microbiology letters, 2005-12, Vol.253 (1), p.155-161</ispartof><rights>2005 Federation of European Microbiological Societies</rights><rights>2005 Federation of European Microbiological Societies 2005</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6745-5bc557fb34472a8c18e8c331be08bf0bbfc8d6fa4ff05d2a077fd4c92f9454df3</citedby><cites>FETCH-LOGICAL-c6745-5bc557fb34472a8c18e8c331be08bf0bbfc8d6fa4ff05d2a077fd4c92f9454df3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.femsle.2005.09.032$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2Fj.femsle.2005.09.032$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17270368$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16242860$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hara-Kudo, Yukiko</creatorcontrib><creatorcontrib>Yoshino, Manabu</creatorcontrib><creatorcontrib>Kojima, Tadashi</creatorcontrib><creatorcontrib>Ikedo, Masanari</creatorcontrib><title>Loop-mediated isothermal amplification for the rapid detection of Salmonella</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Loop-mediated isothermal amplification (LAMP) assay detected
Salmonella within 60
min. The 220 strains of 39 serotypes of
Salmonella subsp.
enterica and 7 strains of
Salmonella enterica subsp.
arizonae were amplified, but not 62 strains of 23 bacterial species other than
Salmonella. The sensitivity of the LAMP assay was found to be >2.2
cfu/test tube using nine serotypes. The specificity was similar to that of a PCR assay, but the sensitivity of LAMP was greater. Both fluorescence and turbidity were able to detect the products in the LAMP assay.
S. enteritidis in a liquid egg sample artificially inoculated with the organism was detected by the LAMP assay at 2.8
cfu/test tube, although negative by PCR assay. These results indicate that the LAMP assay is a rapid, specific and sensitive detection method for
Salmonella.</description><subject>Assaying</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriological Techniques</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Detection</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>Fluorescence</subject><subject>Food Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>LAMP</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Amplification Techniques - methods</subject><subject>Nucleic Acid Amplification Techniques - statistics & numerical data</subject><subject>Polymerase Chain Reaction</subject><subject>Rapid</subject><subject>Salmonella</subject><subject>Salmonella - classification</subject><subject>Salmonella - genetics</subject><subject>Salmonella - isolation & purification</subject><subject>Salmonella enterica</subject><subject>Salmonella enterica - classification</subject><subject>Salmonella enterica - genetics</subject><subject>Salmonella enterica - isolation & purification</subject><subject>Sensitive</subject><subject>Sensitivity</subject><subject>Sensitivity and Specificity</subject><subject>Serotypes</subject><subject>Serotyping</subject><subject>Strains (organisms)</subject><subject>Turbidity</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkk-L1TAUxYMoznP0G4gWxNm1c5PmXzeCDM4oVFyMsw5pmmgebVOTVplvb97rgwEXMqtA8jvnHs4NQq8xVBgwv9xXzo5psBUBYBU0FdTkCdphJmjJGy6foh3UQpYYGnGGXqS0BwBKgD9HZ5gTSiSHHWrbEOZytL3Xi-0Ln8Ly08ZRD4Ue58E7b_Tiw1S4EIv8UkQ9-77o7WLN8T644lYPY5jsMOiX6JnTQ7KvTuc5urv-9P3qc9l-u_ly9bEtDReUlawzjAnX1ZQKoqXB0kpT17izIDsHXeeM7LnT1DlgPdEghOupaYhrKKO9q8_RxeY7x_BrtWlRo0_mkGCyYU0KC0obUpMMvvsH3Ic1TjmbIjVwxiiTB4pulIkhpWidmqMfdbxXGNSha7VXW9fq0LWCRsHR_M3JfO1ygQ-iU7kZeH8CdDJ6cFFPxqcHThABNZeZkxv3xw_2_lHD1fXXFjOWpZebNKzzY1O_3RROB6V_xBzo7pYArgE3jLBj7A8bYfMKf3sbVTLeTib_kZi3rvrg_z_iLwmjyMk</recordid><startdate>200512</startdate><enddate>200512</enddate><creator>Hara-Kudo, Yukiko</creator><creator>Yoshino, Manabu</creator><creator>Kojima, Tadashi</creator><creator>Ikedo, Masanari</creator><general>Elsevier B.V</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>200512</creationdate><title>Loop-mediated isothermal amplification for the rapid detection of Salmonella</title><author>Hara-Kudo, Yukiko ; Yoshino, Manabu ; Kojima, Tadashi ; Ikedo, Masanari</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6745-5bc557fb34472a8c18e8c331be08bf0bbfc8d6fa4ff05d2a077fd4c92f9454df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Assaying</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriological Techniques</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Detection</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Fluorescence</topic><topic>Food Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>LAMP</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Amplification Techniques - methods</topic><topic>Nucleic Acid Amplification Techniques - statistics & numerical data</topic><topic>Polymerase Chain Reaction</topic><topic>Rapid</topic><topic>Salmonella</topic><topic>Salmonella - classification</topic><topic>Salmonella - genetics</topic><topic>Salmonella - isolation & purification</topic><topic>Salmonella enterica</topic><topic>Salmonella enterica - classification</topic><topic>Salmonella enterica - genetics</topic><topic>Salmonella enterica - isolation & purification</topic><topic>Sensitive</topic><topic>Sensitivity</topic><topic>Sensitivity and Specificity</topic><topic>Serotypes</topic><topic>Serotyping</topic><topic>Strains (organisms)</topic><topic>Turbidity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hara-Kudo, Yukiko</creatorcontrib><creatorcontrib>Yoshino, Manabu</creatorcontrib><creatorcontrib>Kojima, Tadashi</creatorcontrib><creatorcontrib>Ikedo, Masanari</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hara-Kudo, Yukiko</au><au>Yoshino, Manabu</au><au>Kojima, Tadashi</au><au>Ikedo, Masanari</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Loop-mediated isothermal amplification for the rapid detection of Salmonella</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2005-12</date><risdate>2005</risdate><volume>253</volume><issue>1</issue><spage>155</spage><epage>161</epage><pages>155-161</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Loop-mediated isothermal amplification (LAMP) assay detected
Salmonella within 60
min. The 220 strains of 39 serotypes of
Salmonella subsp.
enterica and 7 strains of
Salmonella enterica subsp.
arizonae were amplified, but not 62 strains of 23 bacterial species other than
Salmonella. The sensitivity of the LAMP assay was found to be >2.2
cfu/test tube using nine serotypes. The specificity was similar to that of a PCR assay, but the sensitivity of LAMP was greater. Both fluorescence and turbidity were able to detect the products in the LAMP assay.
S. enteritidis in a liquid egg sample artificially inoculated with the organism was detected by the LAMP assay at 2.8
cfu/test tube, although negative by PCR assay. These results indicate that the LAMP assay is a rapid, specific and sensitive detection method for
Salmonella.</abstract><cop>Oxford, UK</cop><pub>Elsevier B.V</pub><pmid>16242860</pmid><doi>10.1016/j.femsle.2005.09.032</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2005-12, Vol.253 (1), p.155-161 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17449232 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Assaying Bacteriological methods and techniques used in bacteriology Bacteriological Techniques Bacteriology Base Sequence Biological and medical sciences Detection DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Fluorescence Food Microbiology Fundamental and applied biological sciences. Psychology Genetics LAMP Microbiology Molecular Sequence Data Nucleic Acid Amplification Techniques - methods Nucleic Acid Amplification Techniques - statistics & numerical data Polymerase Chain Reaction Rapid Salmonella Salmonella - classification Salmonella - genetics Salmonella - isolation & purification Salmonella enterica Salmonella enterica - classification Salmonella enterica - genetics Salmonella enterica - isolation & purification Sensitive Sensitivity Sensitivity and Specificity Serotypes Serotyping Strains (organisms) Turbidity |
| title | Loop-mediated isothermal amplification for the rapid detection of Salmonella |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T17%3A57%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Loop-mediated%20isothermal%20amplification%20for%20the%20rapid%20detection%20of%20Salmonella&rft.jtitle=FEMS%20microbiology%20letters&rft.au=Hara-Kudo,%20Yukiko&rft.date=2005-12&rft.volume=253&rft.issue=1&rft.spage=155&rft.epage=161&rft.pages=155-161&rft.issn=0378-1097&rft.eissn=1574-6968&rft.coden=FMLED7&rft_id=info:doi/10.1016/j.femsle.2005.09.032&rft_dat=%3Cproquest_cross%3E2306554582%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2306554582&rft_id=info:pmid/16242860&rft_oup_id=10.1016/j.femsle.2005.09.032&rft_els_id=S0378109705006683&rfr_iscdi=true |