Loop-mediated isothermal amplification for the rapid detection of Salmonella

Loop-mediated isothermal amplification (LAMP) assay detected Salmonella within 60 min. The 220 strains of 39 serotypes of Salmonella subsp. enterica and 7 strains of Salmonella enterica subsp. arizonae were amplified, but not 62 strains of 23 bacterial species other than Salmonella. The sensitivity...

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Veröffentlicht in:	FEMS microbiology letters 2005-12, Vol.253 (1), p.155-161
Hauptverfasser:	Hara-Kudo, Yukiko, Yoshino, Manabu, Kojima, Tadashi, Ikedo, Masanari
Format:	Artikel
Sprache:	eng
Schlagworte:	Assaying Bacteriological methods and techniques used in bacteriology Bacteriological Techniques Bacteriology Base Sequence Biological and medical sciences Detection DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Fluorescence Food Microbiology Fundamental and applied biological sciences. Psychology Genetics LAMP Microbiology Molecular Sequence Data Nucleic Acid Amplification Techniques - methods Nucleic Acid Amplification Techniques - statistics & numerical data Polymerase Chain Reaction Rapid Salmonella Salmonella - classification Salmonella - genetics Salmonella - isolation & purification Salmonella enterica Salmonella enterica - classification Salmonella enterica - genetics Salmonella enterica - isolation & purification Sensitive Sensitivity Sensitivity and Specificity Serotypes Serotyping Strains (organisms) Turbidity
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Zusammenfassung:	Loop-mediated isothermal amplification (LAMP) assay detected Salmonella within 60 min. The 220 strains of 39 serotypes of Salmonella subsp. enterica and 7 strains of Salmonella enterica subsp. arizonae were amplified, but not 62 strains of 23 bacterial species other than Salmonella. The sensitivity of the LAMP assay was found to be >2.2 cfu/test tube using nine serotypes. The specificity was similar to that of a PCR assay, but the sensitivity of LAMP was greater. Both fluorescence and turbidity were able to detect the products in the LAMP assay. S. enteritidis in a liquid egg sample artificially inoculated with the organism was detected by the LAMP assay at 2.8 cfu/test tube, although negative by PCR assay. These results indicate that the LAMP assay is a rapid, specific and sensitive detection method for Salmonella.
ISSN:	0378-1097 1574-6968
DOI:	10.1016/j.femsle.2005.09.032